Domain · Life Sciences
Most cited papers in Life Sciences
What are the most cited papers in Life Sciences?
The most cited paper in Life Sciences is "PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT" (OliverH. Lowry, NiraJ. Rosebrough, A. Lewis Farr et al., 1951), with 319,095 citations. Next come "Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4" (252,153) and "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding" (225,381).
Showing 50 of 100 papers. Every paper with more than 24,756 citations is listed.
| # | Paper | ||
|---|---|---|---|
| 1 | PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT OliverH. Lowry, NiraJ. Rosebrough, A. Lewis Farr et al. · Journal of Biological Chemistry | Nov 1951 | 319,095 |
| 2 | Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Ulrich K. Laemmli · Nature | Aug 1970 | 252,153 |
| 3 | A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding Mark A. Bradford · Analytical Biochemistry | 7 May 1976 | 225,381 |
| 4 | A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Marion M. Bradford · Analytical Biochemistry | May 1976 | 212,905 |
| 5 | Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method Kenneth J. Livak, Thomas D. Schmittgen · Methods | Dec 2001 | 184,956 |
| 6 | Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 Michael I. Love, Wolfgang K. Huber, Simon Anders · Genome biology | 5 Dec 2014 | 104,335 |
| 7 | Basic local alignment search tool Stephen F. Altschul, Warren R. Gish, Webb Miller et al. · Journal of Molecular Biology | Oct 1990 | 95,803 |
| 8 | U-Net: Convolutional Networks for Biomedical Image Segmentation Olaf Ronneberger, Philipp F. Fischer, Thomas Brox · Lecture notes in computer science | 2015 | 92,751 |
| 9 | A short history of SHELX George M. Sheldrick · Acta Crystallographica Section A Foundations of Crystallography | 21 Dec 2007 | 87,903 |
| 10 | Molecular cloning: A laboratory manual Prescott L. Deininger · Analytical Biochemistry | Apr 1990 | 86,239 |
| 11 | Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Stephen F. Altschul · Nucleic Acids Research | Sep 1997 | 75,032 |
| 12 | Fiji: an open-source platform for biological-image analysis Johannes Schindelin, Ignacio Arganda‐Carreras, Erwin Frise et al. · Nature Methods | 28 Jun 2012 | 73,171 |
| 13 | Trimmomatic: a flexible trimmer for Illumina sequence data Anthony Bolger, Marc Lohse, Björn Usadel · Bioinformatics | Apr 2014 | 71,819 |
| 14 | DNA sequencing with chain-terminating inhibitors Frederick Sanger, S. Nicklen, Alan R. Coulson · Proceedings of the National Academy of Sciences | Dec 1977 | 69,512 |
| 15 | The Sequence Alignment/Map format and SAMtools Heng Li, Bob Handsaker, Alec Wysoker et al. · Bioinformatics | 8 Jun 2009 | 69,061 |
| 16 | VMD: Visual molecular dynamics William F Humphrey Jr, Andrew Dalke, Klaus J. Schulten · Journal of Molecular Graphics | Feb 1996 | 68,537 |
| 17 | A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Toshio Murashige, Folke Skoog · Physiologia Plantarum | Jul 1962 | 67,944 |
| 18 | Hallmarks of Cancer: The Next Generation Douglas Hanahan, Robert A. Weinberg · Cell | Mar 2011 | 67,836 |
| 19 | NIH Image to ImageJ: 25 years of image analysis Caroline A Schneider, Wayne S. Rasband, Kevin W. Eliceiri · Nature Methods | 28 Jun 2012 | 67,169 |
| 20 | Mutation of the mouse klotho gene leads to a syndrome resembling ageing. M Kuro-o, Y Matsumura, H Aizawa et al. · PubMed | 6 Nov 1997 | 66,570 |
| 21 | CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Julie Thompson, Desmond G. Higgins, Toby J. Gibson · Nucleic Acids Research | 1994 | 65,356 |
| 22 | Fast and accurate short read alignment with Burrows–Wheeler transform Heng Li, Richard M. Durbin · Bioinformatics | 18 May 2009 | 63,989 |
| 23 | Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction P CHOMZYNSKI · Analytical Biochemistry | Apr 1987 | 63,261 |
| 24 | Fast gapped-read alignment with Bowtie 2 Ben Langmead, Steven L. Salzberg · Nature Methods | 4 Mar 2012 | 62,825 |
| 25 | The neighbor-joining method: a new method for reconstructing phylogenetic trees. Naruya Saitou, M Nei · Molecular Biology and Evolution | Jul 1987 | 60,643 |
| 26 | Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles Aravind N. Subramanian, Pablo Tamayo, Vamsi Krishna Mootha et al. · Proceedings of the National Academy of Sciences | 30 Sep 2005 | 58,060 |
| 27 | STAR: ultrafast universal RNA-seq aligner Alexander Yu. Dobin, Carrie Davis, Felix Schlesinger et al. · Bioinformatics | 25 Oct 2012 | 58,026 |
| 28 | Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays Tim R. Mosmann · Journal of Immunological Methods | Dec 1983 | 56,216 |
| 29 | Cytoscape: A Software Environment for Integrated Models of Biomolecular Interaction Networks Paul T. Shannon, Andrew Markiel, Owen W. Ozier et al. · Genome Research | Nov 2003 | 55,788 |
| 30 | Research electronic data capture (REDCap)—A metadata-driven methodology and workflow process for providing translational research informatics support Paul A. Harris, ROBERT J. TAYLOR, Robert J. Thielke et al. · Journal of Biomedical Informatics | Oct 2008 | 53,362 |
| 31 | Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China Chaolin Huang et al. · The Lancet | 24 Jan 2020 | 52,028 |
| 32 | MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability Kazutaka Katoh, Daron M. Standley · Molecular Biology and Evolution | 16 Jan 2013 | 50,020 |
| 33 | UCSF Chimera—A visualization system for exploratory research and analysis Eric F. Pettersen, Thomas D. Goddard, Conrad C. Huang et al. · Journal of Computational Chemistry | Jul 2004 | 48,801 |
| 34 | MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0 Koichiro Tamura, Glen Stecher, Daniel S. Peterson et al. · Molecular Biology and Evolution | 16 Oct 2013 | 48,154 |
| 35 | Highly accurate protein structure prediction with AlphaFold John Jumper et al. · Nature | 15 Jul 2021 | 47,486 |
| 36 | Short-Term Effects of Nose-Only Cigarette Smoke Exposure on Glutathione Redox Homeostasis, Cytochrome P450 1A1/2 and Respiratory Enzyme Activities in Mice Tissues Haider Raza, Annie John, Abderrahim Nemmar · Cellular Physiology and Biochemistry | 2013 | 47,289 |
| 37 | MUSCLE: multiple sequence alignment with high accuracy and high throughput R. C. Edgar · Nucleic Acids Research | 8 Mar 2004 | 47,285 |
| 38 | Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Piotr Chomczyński, Nicoletta Sacchi · Analytical Biochemistry | Apr 1987 | 47,055 |
| 39 | MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets Sudhir Kumar, Glen Stecher, Koichiro Tamura · Molecular Biology and Evolution | 22 Mar 2016 | 46,273 |
| 40 | edgeR : a Bioconductor package for differential expression analysis of digital gene expression data Mark D. Robinson, Davis James McCarthy, Gordon K. Smyth · Bioinformatics | 11 Nov 2009 | 45,449 |
| 41 | Gene Ontology: tool for the unification of biology Michael Ashburner, Catherine A. Ball, Judith A. Blake et al. · Nature Genetics | May 2000 | 45,425 |
| 42 | limma powers differential expression analyses for RNA-sequencing and microarray studies Matthew E. Ritchie, Belinda Phipson, Di Wu et al. · Nucleic Acids Research | 20 Jan 2015 | 44,530 |
| 43 | Collective dynamics of ‘small-world’ networks Duncan J. Watts, Steven H. Strogatz · Nature | Jun 1998 | 43,707 |
| 44 | A power primer. Jacob Cohen · Psychological Bulletin | 1992 | 42,833 |
| 45 | CONFIDENCE LIMITS ON PHYLOGENIES: AN APPROACH USING THE BOOTSTRAP Joseph Felsenstein · Evolution | Jul 1985 | 41,763 |
| 46 | The Protein Data Bank Helen Miriam Berman · Nucleic Acids Research | 2000 | 40,564 |
| 47 | MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods Koichiro Tamura, Daniel Gunnar Peterson, Nora Peterson et al. · Molecular Biology and Evolution | 4 May 2011 | 40,453 |
| 48 | KEGG: Kyoto Encyclopedia of Genes and Genomes Minoru I. Kanehisa · Nucleic Acids Research | 2000 | 40,289 |
| 49 | SciPy 1.0: fundamental algorithms for scientific computing in Python Pauli Virtanen et al. · Nature Methods | 3 Feb 2020 | 40,161 |
| 50 | clusterProfiler: an R Package for Comparing Biological Themes Among Gene Clusters Guangchuang Yu, Li-Gen Wang, Yanyan Han et al. · OMICS A Journal of Integrative Biology | 28 Mar 2012 | 39,704 |
Citations are OpenAlex's own counts, from its 2026-09-23 snapshot, so the ranking can differ from Google Scholar or Web of Science. A paper is listed for a field when OpenAlex files it under one of the field's topics; retracted papers and papers whose citation count cannot be traced to citing works are left out, and a preprint and the article it became are shown once. Release dates are OpenAlex's: where a source gave only a month or a year, that is what is shown. How the lists are chosen.