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Most cited papers in Agricultural and Biological Sciences

What are the most cited papers in Agricultural and Biological Sciences?

The most cited paper in Agricultural and Biological Sciences is "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding" (Mark A. Bradford, 1976), with 225,381 citations. Next come "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding" (212,905) and "A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures" (67,944).

Showing 50 of 100 papers. Every paper with more than 7,944 citations is listed.

Most cited papers, sortable by citations and release date
#Paper
1 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding Mark A. Bradford · Analytical Biochemistry 7 May 1976 225,381
2 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Marion M. Bradford · Analytical Biochemistry May 1976 212,905
3 A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Toshio Murashige, Folke Skoog · Physiologia Plantarum Jul 1962 67,944
4 A power primer. Jacob Cohen · Psychological Bulletin 1992 42,833
5 DADA2: High-resolution sample inference from Illumina amplicon data Benjamin J. Callahan, Paul J. McMurdie, Michael Rosen et al. · Nature Methods Open access 23 May 2016 37,958
6 Statistical power analyses using G*Power 3.1: Tests for correlation and regression analyses Franz Faul, Edgar Erdfelder, Axel Buchner et al. · Behavior Research Methods Open access Nov 2009 37,775
7 MicroRNAs David P. Bartel · Cell Open access 2004 35,138
8 Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction Hiroshi Ohkawa, Nobuko Ohishi, Kunio Yagi · Analytical Biochemistry Jun 1979 29,425
9 MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0 Koichiro Tamura, Joel T. Dudley, M Nei et al. · Molecular Biology and Evolution Open access 18 Apr 2007 28,894
10 Methods for Dietary Fiber, Neutral Detergent Fiber, and Nonstarch Polysaccharides in Relation to Animal Nutrition Peter J. Van Soest, James B. Robertson, B.A. Lewis · Journal of Dairy Science Open access Oct 1991 28,661
11 IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood Phylogenies Lam-Tung Nguyen, Heiko A. Schmidt, Arndt von Haeseler et al. · Molecular Biology and Evolution Open access 3 Nov 2014 28,418
12 RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome Bo Li, Colin N. Dewey · BMC Bioinformatics Open access 4 Aug 2011 24,027
13 A pneumonia outbreak associated with a new coronavirus of probable bat origin Peng Zhou et al. · Nature Open access 3 Feb 2020 23,429
14 Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study Nanshan Chen, Min Zhou, Xuan Dong et al. · The Lancet Open access 30 Jan 2020 22,766
15 Floral dip: a simplified method for Agrobacterium ‐mediated transformation of Arabidopsis thaliana Steven John Clough, Andrew F. Bent · The Plant Journal Open access Dec 1998 22,756
16 Rapid determination of free proline for water-stress studies Lynn S. Bates, R. P. Waldren, Iwan D. Teare · Plant and Soil Aug 1973 21,112
17 The Effect of Riding as an Alternative Treatment for Children with Cerebral Palsy: A Systematic Review and Meta-Analysis Guoqin Wang, Ruiqin Ma, Guangwei Qiao et al. · Integrative Medicine International ReviewOpen access 2 Apr 2015 18,689
18 Free radicals in biology and medicine J. M. C. Gutteridge · General Pharmacology The Vascular System 1991 17,218
19 A Simple, Fast, and Accurate Algorithm to Estimate Large Phylogenies by Maximum Likelihood Stéphane Guindon, Olivier Gascuel · Systematic Biology Open access Oct 2003 17,109
20 genalex 6: genetic analysis in Excel. Population genetic software for teaching and research Rod Peakall, Peter E. Smouse · Molecular Ecology Notes 29 Sep 2005 16,827
21 Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype Daehwan Kim, Joseph M. Paggi, Chanhee Park et al. · Nature Biotechnology Open access Aug 2019 16,558
22 Table 1 in A new Gammarus species from Xinjiang Uygur Autonomous Region (China) with a key to Xinjiang freshwater gammarids (Crustacea, Amphipoda, Gammaridae) Zhang, Kui, Wang, Jun, Ge, Yihao et al. · PubMed Open access 25 Mar 2022 15,805
23 UCHIME improves sensitivity and speed of chimera detection R. C. Edgar, Brian J. Haas, José Carlos Clemente et al. · Bioinformatics Open access 23 Jun 2011 15,726
24 GENEPOP (Version 1.2): Population Genetics Software for Exact Tests and Ecumenicism Michel Raymond, François Rousset · Journal of Heredity May 1995 15,620
25 ggplot2: Elegant Graphics for Data Analysis Cedric E. Ginestet · Journal of the Royal Statistical Society Series A (Statistics in Society) 2011 14,905
26 Multivariate Data Analysis Xianggui Qu · Technometrics Feb 2007 14,841
27 A comprehensive set of sequence analysis programs for the VAX John Devereux, Paul E. Haeberli, Oliver Smithies · Nucleic Acids Research Open access 1984 14,432
28 TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions Daehwan Kim, Geo M. Pertea, Cole Trapnell et al. · Genome biology Open access 25 Apr 2013 13,857
29 Mechanisms of Salinity Tolerance Rana Munns, Mark A. Tester · Annual Review of Plant Biology Review 7 Feb 2008 13,850
30 Estimates of the Regression Coefficient Based on Kendall's Tau Pranab Kumar Sen · Journal of the American Statistical Association Dec 1968 13,754
31 A new method for non‐parametric multivariate analysis of variance Marti Jane Anderson · Austral Ecology Feb 2001 13,523
32 The plant immune system Jonathan D. G. Jones, Jeffery L. Dangl · Nature Open access Nov 2006 13,434
33 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers John Garnett Williams, Anne R. Kubelik, Kenneth J. Livak et al. · Nucleic Acids Research Open access 1990 13,158
34 Antioxidant Determinations by the Use of a Stable Free Radical Marsden S. Blois · Nature Apr 1958 13,038
35 An extraction method for measuring soil microbial biomass C Eric D. Vance, Philip Charles Brookes, David Stewart Jenkinson · Soil Biology and Biochemistry 1987 13,020
36 A new coronavirus associated with human respiratory disease in China Fan Wu et al. · Nature Open access 3 Feb 2020 12,967
37 STRUCTURE HARVESTER: a website and program for visualizing STRUCTURE output and implementing the Evanno method Dent A. Earl, Bridgett M. vonHoldt · Conservation Genetics Resources 12 Oct 2011 12,797
38 Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding Roujian Lu et al. · The Lancet Open access 30 Jan 2020 12,621
39 Use of Ranks in One-Criterion Variance Analysis William Kruskal, W. Allen Wallis · Journal of the American Statistical Association Dec 1952 12,511
40 The Application of Electronic Computers to Factor Analysis Henry F. Kaiser · Educational and Psychological Measurement Apr 1960 12,470
41 Rapid isolation of high molecular weight plant DNA Michael G. Murray, William F. Thompson · Nucleic Acids Research Open access 1980 12,462
42 Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation Ivica Letunić, Peer Bork · Nucleic Acids Research Open access 12 Apr 2021 12,232
43 Circos: An information aesthetic for comparative genomics Martin I. Krzywinski, Jacqueline E. Schein, İnanç Birol et al. · Genome Research Open access 18 Jun 2009 12,144
44 Conserved Seed Pairing, Often Flanked by Adenosines, Indicates that Thousands of Human Genes are MicroRNA Targets Benjamin P. Lewis, Christopher B. Burge, David P. Bartel · Cell Open access 2005 12,048
45 QUAST: quality assessment tool for genome assemblies Alexey A. Gurevich, Vladislav Saveliev, Nikolay Vyahhi et al. · Bioinformatics Open access 19 Feb 2013 11,980
46 Red and photographic infrared linear combinations for monitoring vegetation Compton J. Tucker · Remote Sensing of Environment Open access May 1979 11,851
47 Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants Sarvajeet Singh Gill, Narendra Tuteja · Plant Physiology and Biochemistry 16 Sep 2010 11,846
48 REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal Transduction Klaus Apel, Heribert Hirt · Annual Review of Plant Biology Review 29 Apr 2004 11,793
49 The Human Genome Browser at UCSC W. James Kent, Charles W. Sugnet, Terrence S. Furey et al. · Genome Research Open access 16 May 2002 10,998
50 Oxidative stress, antioxidants and stress tolerance Ron Mittler · Trends in Plant Science Review Sep 2002 10,890

Citations are OpenAlex's own counts, from its 2026-09-23 snapshot, so the ranking can differ from Google Scholar or Web of Science. A paper is listed for a field when OpenAlex files it under one of the field's topics; retracted papers and papers whose citation count cannot be traced to citing works are left out, and a preprint and the article it became are shown once. Release dates are OpenAlex's: where a source gave only a month or a year, that is what is shown. How the lists are chosen.