| 1 |
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research
Jan Van Deun et al. · Nature Methods
|
28 Feb 2017 |
1,345 |
| 2 |
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals
Ina Poser et al. · Nature Methods
Open access |
6 Apr 2008 |
637 |
| 3 |
Full-length RecE enhances linear-linear homologous recombination and facilitates direct cloning for bioprospecting
Jun Fu, Xiaoying Bian, Shengbaio Hu et al. · Nature Biotechnology
|
29 Apr 2012 |
454 |
| 4 |
Techniques: Recombinogenic engineering–new options for cloning and manipulating DNA
Joep P. P. Muyrers, Youming Zhang, Adrian Francis Stewart · Trends in Biochemical Sciences
Review |
May 2001 |
259 |
| 5 |
Novel expression hosts for complex secondary metabolite megasynthetases: Production of myxochromide in the thermopilic isolate Corallococcus macrosporus GT-2
Olena Perlova, Klaus Gerth, Silvia Kuhlmann et al. · Microbial Cell Factories
Open access |
6 Jan 2009 |
252 |
| 6 |
Involvement of G-quadruplex regions in mammalian replication origin activity
Paulina Prorok, Marie Artufel, Antoine Aze et al. · Nature Communications
Open access |
22 Jul 2019 |
207 |
| 7 |
A recombineering pipeline for functional genomics applied to Caenorhabditis elegans
Mihail Sarov, Susan M. Schneider, Andrei Pozniakovski et al. · Nature Methods
Open access |
21 Sep 2006 |
206 |
| 8 |
Heterologous Expression of a Myxobacterial Natural Products Assembly Line in Pseudomonads via Red/ET Recombineering
Silke C. Wenzel, Frank B. Gross, Youming Zhang et al. · Chemistry & Biology
Open access |
Mar 2005 |
183 |
| 9 |
Successful engraftment of gene-corrected hematopoietic stem cells in non-conditioned patients with Fanconi anemia
Paula Rı́o et al. · Nature Medicine
|
Sep 2019 |
180 |
| 10 |
Myelodysplastic syndrome unrelated to lentiviral vector in a patient treated with gene therapy for sickle cell disease
Matthew M. Hsieh, Melissa A. Bonner, Francis John Pierciey et al. · Blood Advances
Open access |
12 May 2020 |
152 |
| 11 |
Improved seamless mutagenesis by recombineering using ccdB for counterselection
Hailong Wang, Xiaoying Bian, Liqiu Xia et al. · Nucleic Acids Research
Open access |
24 Dec 2013 |
150 |
| 12 |
Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition
Jun Fu, Silke C. Wenzel, Olena Perlova et al. · Nucleic Acids Research
Open access |
13 Aug 2008 |
149 |
| 13 |
An Improved Recombineering Approach by Adding RecA to λ Red Recombination
Junping Wang, Mihail Sarov, Jeanette M. J. Rientjes et al. · Molecular Biotechnology
|
28 Dec 2005 |
144 |
| 14 |
Phage annealing proteins promote oligonucleotide-directed mutagenesis in Escherichia coli and mouse ES cells
Youming Zhang, Joep P. P. Muyrers, Jeanette M. J. Rientjes et al. · BMC Molecular Biology
Open access |
16 Jan 2003 |
134 |
| 15 |
Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles
Giuseppe Testa, Youming Zhang, Kristina Vintersten et al. · Nature Biotechnology
|
10 Mar 2003 |
131 |
| 16 |
Automated Yeast Two-hybrid Screening for Nuclear Receptor-interacting Proteins
M. Albers, Harald D. Kranz, Ingo Kober et al. · Molecular & Cellular Proteomics
Open access |
16 Dec 2004 |
127 |
| 17 |
Lovo‐cel gene therapy for sickle cell disease: Treatment process evolution and outcomes in the initial groups of the HGB ‐206 study
Julie Kanter, Alexis A. Thompson, Francis John Pierciey et al. · American Journal of Hematology
Open access |
26 Sep 2022 |
126 |
| 18 |
Single-stranded heteroduplex intermediates in λ Red homologous recombination
Marcello Maresca, Axel Erler, Jun Fu et al. · BMC Molecular Biology
Open access |
2010 |
126 |
| 19 |
Long-term outcomes of lentiviral gene therapy for the β-hemoglobinopathies: the HGB-205 trial
Elisa Magrin et al. · Nature Medicine
|
2022 |
105 |
| 20 |
Common schizophrenia risk variants are enriched in open chromatin regions of human glutamatergic neurons
Mads E. Hauberg, Jordi Creus‐Muncunill, Jaroslav M. Bendl et al. · Nature Communications
Open access |
4 Nov 2020 |
99 |
| 21 |
Reconstitution of the Myxothiazol Biosynthetic Gene Cluster by Red/ET Recombination and Heterologous Expression in Myxococcus xanthus
Olena Perlova, Jun Fu, Silvia Kuhlmann et al. · Applied and Environmental Microbiology
Open access |
Dec 2006 |
90 |
| 22 |
Metabolic Engineering of Pseudomonas putida for Methylmalonyl-CoA Biosynthesis to Enable Complex Heterologous Secondary Metabolite Formation
Frank B. Gross, Michael W. Ring, Olena Perlova et al. · Chemistry & Biology
Open access |
Dec 2006 |
76 |
| 23 |
Simple generation of site-directed point mutations in the Escherichia coli chromosome using Red®/ET® Recombination
Ralf Heermann, Tim Zeppenfeld, Kirsten Jung · Microbial Cell Factories
Open access |
2008 |
73 |
| 24 |
Direct Cloning, Genetic Engineering, and Heterologous Expression of the Syringolin Biosynthetic Gene Cluster in E. coli through Red/ET Recombineering
Xiaoying Bian, Fan Huang, Francis Stewart et al. · ChemBioChem
|
31 Jul 2012 |
72 |
| 25 |
Prediction of clinical diagnosis of Alzheimer’s disease, vascular, mixed, and all-cause dementia by a polygenic risk score and APOE status in a community-based cohort prospectively followed over 17 years
Hannah R. Stocker, Laura Perna, Korbinian Weigl et al. · Molecular Psychiatry
Open access |
13 May 2020 |
71 |
| 26 |
AAV vector-mediated in vivo reprogramming into pluripotency
Elena Senís, Lluc Mosteiro, Stefan Wilkening et al. · Nature Communications
Open access |
3 Jul 2018 |
68 |
| 27 |
Heterologous Expression and Genetic Engineering of the Tubulysin Biosynthetic Gene Cluster Using Red/ET Recombineering and Inactivation Mutagenesis
Yi Chai, Shiping Shan, Kira J. Weissman et al. · Chemistry & Biology
Open access |
Mar 2012 |
65 |
| 28 |
High-efficiency counterselection recombineering for site-directed mutagenesis in bacterial artificial chromosomes
Alexander W. Bird, Axel Erler, Jun Fu et al. · Nature Methods
Open access |
4 Dec 2011 |
62 |
| 29 |
Escherichia Coli Strains with Promoter Libraries Constructed by Red/ET Recombination Pave the Way for Transcriptional Fine-Tuning
Stephan Braatsch, Søren Helmark, Harald D. Kranz et al. · BioTechniques
Open access |
Sep 2008 |
58 |
| 30 |
Luminmycins A–C, Cryptic Natural Products from Photorhabdus luminescens Identified by Heterologous Expression in Escherichia coli
Xiaoying Bian, Alberto Plaza, Youming Zhang et al. · Journal of Natural Products
|
21 Aug 2012 |
56 |
| 31 |
Regulatory systems controlling motility and gene transfer agent production and release in Rhodobacter capsulatus
Ryan G. Mercer, Matthew Quinlan, Alexandra R. Rose et al. · FEMS Microbiology Letters
|
24 Mar 2012 |
50 |
| 32 |
Long-Term Follow-Up of Hematopoietic Stem-Cell Gene Therapy for Cerebral Adrenoleukodystrophy
Pierre F Bougneres, Salima Hacein‐Bey‐Abina, Ivan Labik et al. · Human Gene Therapy
|
Apr 2021 |
45 |
| 33 |
Investigating the mechanisms of ribonucleotide excision repair in Escherichia coli
Alexandra Vaisman, John P. McDonald, Stephan Noll et al. · Mutation research. Fundamental and molecular mechanisms of mutagenesis
Open access |
2 Feb 2014 |
44 |
| 34 |
Generation and Clinical Application of Gene-Modified Autologous Epidermal Sheets in Netherton Syndrome: Lessons Learned from a Phase 1 Trial
Wei‐Li Di et al. · Human Gene Therapy
Open access |
10 Jul 2019 |
41 |
| 35 |
Familial risks between Graves disease and Hashimoto thyroiditis and other autoimmune diseases in the population of Sweden
Hauke Thomsen, Xinjun Li, Kristina Sundquist et al. · Journal of Translational Autoimmunity
Open access |
2020 |
37 |
| 36 |
A case of T-cell acute lymphoblastic leukemia in retroviral gene therapy for ADA-SCID
Daniela Cesana et al. · Nature Communications
Open access |
30 Apr 2024 |
34 |
| 37 |
Modification of the Genome of Rhodobacter sphaeroides and Construction of Synthetic Operons
Paul R. Jaschke, Rafael G. Saer, Stephan Noll et al. · Methods in enzymology on CD-ROM/Methods in enzymology
Book chapter |
2011 |
34 |
| 38 |
Therapeutic expression of human clotting factors IX and × following adeno‐associated viral vector‐mediated intrauterine gene transfer in early‐gestation fetal macaques
Jerry Kok Yen Chan, Irene Gil-Fariña, Nuryanti Binti Johana et al. · The FASEB Journal
Open access |
5 Dec 2018 |
33 |
| 39 |
Efficient Nuclease-Directed Integration of Lentivirus Vectors into the Human Ribosomal DNA Locus
Diana Schenkwein, Saira Afzal, Alisa Nousiainen et al. · Molecular Therapy
Open access |
22 May 2020 |
32 |
| 40 |
Haematopoietic gene therapy of non-conditioned patients with Fanconi anaemia-A: results from open-label phase 1/2 (FANCOLEN-1) and long-term clinical trials
Paula Rı́o et al. · The Lancet
|
Dec 2024 |
31 |
| 41 |
ET Recombination: DNA Engineering Using Homologous Recombination in E. coli
Joep P. P. Muyrers, Youming Zhang, Vladimı́r Beneš et al. · Humana Press eBooks
Book chapter |
15 Mar 2004 |
30 |
| 42 |
BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes
Giuseppe Testa, Kristina Vintersten, Youming Zhang et al. · Humana Press eBooks
Book chapter |
15 Mar 2004 |
26 |
| 43 |
Development and characterization of Escherichia coli triple reporter strains for investigation of population heterogeneity in bioprocesses
Anna‐Lena Heins, Jan Reyelt, Marlen Schmidt et al. · Microbial Cell Factories
Open access |
28 Jan 2020 |
24 |
| 44 |
Multiethnic genome‐wide association study of differentiated thyroid cancer in the EPITHYR consortium
Thérèse Truong et al. · International Journal of Cancer
|
3 Feb 2021 |
22 |
| 45 |
VSeq-Toolkit: Comprehensive Computational Analysis of Viral Vectors in Gene Therapy
Saira Afzal, Raffaele Fronza, Manfred Schmidt · Molecular Therapy — Methods & Clinical Development
Open access |
30 Mar 2020 |
18 |
| 46 |
High-level expression, purification and antibacterial activity of bovine lactoferricin and lactoferrampin in Photorhabdus luminescens
Zhiru Tang, Youming Zhang, Adrian Francis Stewart et al. · Protein Expression and Purification
|
26 May 2010 |
18 |
| 47 |
Resolution of Serious Vaso-Occlusive Pain Crises and Reduction in Patient-Reported Pain Intensity: Results from the Ongoing Phase 1/2 HGB-206 Group C Study of LentiGlobin for Sickle Cell Disease (bb1111) Gene Therapy
Alexis A. Thompson, Mark C. Walters, Markus Y. Mapara et al. · Blood
|
5 Nov 2020 |
15 |
| 48 |
The balance between the intronic miR-342 and its host gene Evl determines hematopoietic cell fate decision
Friederike Herbst, Tonio Johannes Lukas Lang, Elias Eckert et al. · Leukemia
Open access |
21 May 2021 |
14 |
| 49 |
Mapping Active Gene-Regulatory Regions in Human Repopulating Long-Term HSCs
Peer Wünsche, Elias Eckert, Tim Holland‐Letz et al. · Cell stem cell
Open access |
Jul 2018 |
14 |
| 50 |
HIV- 1 lentivirus tethering to the genome is associated with transcription factor binding sites found in genes that favour virus survival
Saqlain Suleman, Annette M. Payne, Johnathan Bowden et al. · Gene Therapy
Open access |
5 May 2022 |
13 |