Most cited papers in Bacterial Genetics and Biotechnology
What are the most cited papers in Bacterial Genetics and Biotechnology?
The most cited paper in Bacterial Genetics and Biotechnology is "DNA sequencing with chain-terminating inhibitors" (Frederick Sanger, S. Nicklen, Alan R. Coulson, 1977), with 69,512 citations. Next come "MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability" (50,020) and "Prokka: rapid prokaryotic genome annotation" (20,396).
Showing 50 of 100 papers. Every paper with more than 1,918 citations is listed.
| # | Paper | ||
|---|---|---|---|
| 1 | DNA sequencing with chain-terminating inhibitors Frederick Sanger, S. Nicklen, Alan R. Coulson · Proceedings of the National Academy of Sciences | Dec 1977 | 69,512 |
| 2 | MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability Kazutaka Katoh, Daron M. Standley · Molecular Biology and Evolution | 16 Jan 2013 | 50,020 |
| 3 | Prokka: rapid prokaryotic genome annotation Torsten Seemann · Bioinformatics | 18 Mar 2014 | 20,396 |
| 4 | One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences | 30 May 2000 | 15,367 |
| 5 | Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors Celeste Yanisch-Perron, Jeffrey Vieira, Joachim W. Messing · Gene | 1985 | 15,189 |
| 6 | A rapid alkaline extraction procedure for screening recombinant plasmid DNA H. Chaim Birnboim, Janine Doly · Nucleic Acids Research | 24 Nov 1979 | 14,900 |
| 7 | The RAST Server: Rapid Annotations using Subsystems Technology Ramy Karam Aziz et al. · BMC Genomics | 8 Feb 2008 | 12,071 |
| 8 | Studies on transformation of Escherichia coli with plasmids Douglas Hanahan · Journal of Molecular Biology | Jun 1983 | 11,339 |
| 9 | Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase Craig Tuerk, Larry Gold · Science | 3 Aug 1990 | 10,507 |
| 10 | The Phyre2 web portal for protein modeling, prediction and analysis Lawrence A. Kelley, Stefans Mezulis, Christopher M. Yates et al. · Nature Protocols | 7 May 2015 | 10,412 |
| 11 | RNA-Guided Human Genome Engineering via Cas9 Prashant Mali, Luhan Yang, Kevin M. Esvelt et al. · Science | 4 Jan 2013 | 9,548 |
| 12 | Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection Tomoya Baba, Takeshi Ara, Miki Launois Hasegawa et al. · Molecular Systems Biology | 21 Feb 2006 | 8,362 |
| 13 | The Complete Genome Sequence of Escherichia coli K-12 Frederick R. Blattner, Guy Plunkett, Craig A. Bloch et al. · Science | 5 Sep 1997 | 7,594 |
| 14 | Identification of the Cystic Fibrosis Gene: Cloning and Characterization of Complementary DNA John R. Riordan, Johanna M. Rommens, Bat-Sheva Kerem et al. · Science | 8 Sep 1989 | 7,480 |
| 15 | A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria Reinhard Simon, U. B. Priefer, Alfred Pühler · Nature Biotechnology | Nov 1983 | 7,350 |
| 16 | SIFT: predicting amino acid changes that affect protein function P.C. Ng · Nucleic Acids Research | 24 Jun 2003 | 6,991 |
| 17 | Genetic regulatory mechanisms in the synthesis of proteins François Jacob, Jacques Monod · Journal of Molecular Biology | Jun 1961 | 6,659 |
| 18 | Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes F. William Studier, Barbara A. Moffatt · Journal of Molecular Biology | May 1986 | 6,437 |
| 19 | Protein production by auto-induction in high-density shaking cultures F. William Studier · Protein Expression and Purification | 8 Feb 2005 | 6,238 |
| 20 | Roary: rapid large-scale prokaryote pan genome analysis Andrew J. Page, Carla A. Cummins, Martin B. Hunt et al. · Bioinformatics | 20 Jul 2015 | 6,188 |
| 21 | Development and Applications of CRISPR-Cas9 for Genome Engineering Patrick D. Hsu, Eric S. Lander, Feng Zhang · Cell | Jun 2014 | 5,993 |
| 22 | Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae Mark S. Longtine, Amos Mckenzie, Douglas James DeMarini et al. · Yeast | 4 Dec 1998 | 5,582 |
| 23 | Quorum Sensing in Bacteria Melissa B. Miller, Bonnie L. Bassler · Annual Review of Microbiology | Oct 2001 | 5,167 |
| 24 | Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter Luz‐Maria Guzman, Dominique Belin, Michael J. Carson et al. · Journal of Bacteriology | Jul 1995 | 5,079 |
| 25 | Precise Measurement of the G+C Content of Deoxyribonucleic Acid by High-Performance Liquid Chromatography M. K. M. Mesbah, USHA PREMACHANDRAN, William B. Whitman · International Journal of Systematic Bacteriology | Apr 1989 | 4,557 |
| 26 | Lipopolysaccharide Endotoxins Christian R.H. Raetz, Chris Whitfield · Annual Review of Biochemistry | Jun 2002 | 4,420 |
| 27 | Molecular Basis of Bacterial Outer Membrane Permeability Revisited Hiroshi Nikaido · Microbiology and Molecular Biology Reviews | Dec 2003 | 4,351 |
| 28 | Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing Steven Henikoff · Gene | Jun 1984 | 4,214 |
| 29 | Functional Characterization of the S. cerevisiae Genome by Gene Deletion and Parallel Analysis Elizabeth A. Winzeler et al. · Science | 6 Aug 1999 | 4,050 |
| 30 | QUORUM SENSING: Cell-to-Cell Communication in Bacteria Christopher M. Waters, Bonnie L. Bassler · Annual Review of Cell and Developmental Biology | 28 Jun 2005 | 4,030 |
| 31 | Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure David H. Mathews, Jeffrey Sabina, Michael Zuker et al. · Journal of Molecular Biology | May 1999 | 3,877 |
| 32 | Capturing Chromosome Conformation Job Dekker, Karsten Rippe, Martijn Dekker et al. · Science | 15 Feb 2002 | 3,876 |
| 33 | A complementation analysis of the restriction and modification of DNA in Escherichia coli Herbert W. Boyer, Daisy Roulland-Dussoix · Journal of Molecular Biology | May 1969 | 3,863 |
| 34 | The Bacterial Cell Envelope Thomas J. Silhavy, Daniel E. Kahne, Scott S. Walker · Cold Spring Harbor Perspectives in Biology | 14 Apr 2010 | 3,848 |
| 35 | Maturation of the head of bacteriophage T4 Ulrich K. Laemmli, Manuel Favre · Journal of Molecular Biology | Nov 1973 | 3,737 |
| 36 | The complete genome sequence of the Gram-positive bacterium Bacillus subtilis Frank Kunst et al. · Nature | Nov 1997 | 3,720 |
| 37 | Two-Component Signal Transduction Ann Marie Stock, Victoria Robinson, Paul N. Goudreau · Annual Review of Biochemistry | Jun 2000 | 3,702 |
| 38 | BLAST Ring Image Generator (BRIG): simple prokaryote genome comparisons Nabil-Fareed Alikhan, Nicola K. Petty, Nouri L. Ben Zakour et al. · BMC Genomics | 8 Aug 2011 | 3,494 |
| 39 | Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing Frederick Sanger, Alan R. Coulson, B. G. Barrell et al. · Journal of Molecular Biology | Oct 1980 | 3,465 |
| 40 | The Involvement of Cell-to-Cell Signals in the Development of a Bacterial Biofilm David G. Davies, Matthew R. Parsek, James P. Pearson et al. · Science | 10 Apr 1998 | 3,416 |
| 41 | Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes Michael E. Kovach, Philip H. Elzer, D. Steven Hill et al. · Gene | Dec 1995 | 3,408 |
| 42 | Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans David H. Figurski, Donald R. Helinski · Proceedings of the National Academy of Sciences | Apr 1979 | 3,306 |
| 43 | A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Stanley Tabor, Carolyn C. Richardson · Proceedings of the National Academy of Sciences | Feb 1985 | 3,179 |
| 44 | 4 Lipid and Cell-Wall Analysis in Bacterial Systematics Kazuo Komagata, Ken‐ichiro Suzuki · Methods in microbiology | 1988 | 3,129 |
| 45 | A methodology for performing global uncertainty and sensitivity analysis in systems biology Simeone Marino, Ian B. Hogue, Christian J. Ray et al. · Journal of Theoretical Biology | 21 Apr 2008 | 3,092 |
| 46 | Network motifs in the transcriptional regulation network of Escherichia coli Shai S. Shen-Orr, Ron Milo, Shmoolik Mangan et al. · Nature Genetics | 22 Apr 2002 | 3,075 |
| 47 | A Common Mechanism of Cellular Death Induced by Bactericidal Antibiotics Michael A. Kohanski, Daniel J. Dwyer, Boris Hayete et al. · Cell | Sep 2007 | 3,035 |
| 48 | Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators W. Claiborne Fuqua, Stephen C. Winans, Everett Peter Greenberg · Journal of Bacteriology | 1994 | 2,956 |
| 49 | A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments Joachim W. Messing, Jeffrey Vieira · Gene | Oct 1982 | 2,943 |
| 50 | New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites R. Daniel Gietz, Akio Sugino · Gene | Dec 1988 | 2,922 |
Citations are OpenAlex's own counts, from its 2026-09-23 snapshot, so the ranking can differ from Google Scholar or Web of Science. A paper is listed for a field when OpenAlex files it under one of the field's topics; retracted papers and papers whose citation count cannot be traced to citing works are left out, and a preprint and the article it became are shown once. Release dates are OpenAlex's: where a source gave only a month or a year, that is what is shown. How the lists are chosen.