Most cited papers in Genetics, Bioinformatics, and Biomedical Research
What are the most cited papers in Genetics, Bioinformatics, and Biomedical Research?
The most cited paper in Genetics, Bioinformatics, and Biomedical Research is "Molecular cloning: A laboratory manual" (Prescott L. Deininger, 1990), with 86,239 citations. Next come "Fiji: an open-source platform for biological-image analysis" (73,171) and "Trimmomatic: a flexible trimmer for Illumina sequence data" (71,819).
Showing 50 of 100 papers. Every paper with more than 1,439 citations is listed.
| # | Paper | ||
|---|---|---|---|
| 1 | Molecular cloning: A laboratory manual Prescott L. Deininger · Analytical Biochemistry | Apr 1990 | 86,239 |
| 2 | Fiji: an open-source platform for biological-image analysis Johannes Schindelin, Ignacio Arganda‐Carreras, Erwin Frise et al. · Nature Methods | 28 Jun 2012 | 73,171 |
| 3 | Trimmomatic: a flexible trimmer for Illumina sequence data Anthony Bolger, Marc Lohse, Björn Usadel · Bioinformatics | Apr 2014 | 71,819 |
| 4 | NIH Image to ImageJ: 25 years of image analysis Caroline A Schneider, Wayne S. Rasband, Kevin W. Eliceiri · Nature Methods | 28 Jun 2012 | 67,169 |
| 5 | KEGG: Kyoto Encyclopedia of Genes and Genomes Minoru I. Kanehisa · Nucleic Acids Research | 2000 | 40,289 |
| 6 | SciPy 1.0: fundamental algorithms for scientific computing in Python Pauli Virtanen et al. · Nature Methods | 3 Feb 2020 | 40,161 |
| 7 | The Structure of Scientific Revolutions Thomas Kühn, Richard Schlegel · Physics Today | Apr 1963 | 35,485 |
| 8 | Geneious Basic: An integrated and extendable desktop software platform for the organization and analysis of sequence data Matthew D. Kearse, Richard Moir, Amy Swango Wilson et al. · Bioinformatics | 27 Apr 2012 | 21,229 |
| 9 | Prokka: rapid prokaryotic genome annotation Torsten Seemann · Bioinformatics | 18 Mar 2014 | 20,396 |
| 10 | Primer3 on the WWW for General Users and for Biologist Programmers Steve Rozen, Helen Skaletsky · Humana Press eBooks | 14 Nov 2003 | 15,110 |
| 11 | Molecular Structure of Nucleic Acids: A Structure for Deoxyribose Nucleic Acid James Dewey Watson, Francis Harry Compton Crick · Nature | 25 Apr 1953 | 13,452 |
| 12 | WebLogo: A Sequence Logo Generator: Figure 1 Gavin E. Crooks, Gary C. Hon, John‐Marc Chandonia et al. · Genome Research | Jun 2004 | 13,238 |
| 13 | Bioconductor: open software development for computational biology and bioinformatics Robert C. Gentleman et al. · Genome biology | 15 Sep 2004 | 12,632 |
| 14 | Creating the CIPRES Science Gateway for inference of large phylogenetic trees Mark A. Miller, Wayne Pfeiffer, Terri Schwartz | Nov 2010 | 11,502 |
| 15 | STRING v10: protein–protein interaction networks, integrated over the tree of life Damian Szklarczyk, Andrea Franceschini, Stefan Wyder et al. · Nucleic Acids Research | 28 Oct 2014 | 11,220 |
| 16 | Database resources of the National Center for Biotechnology Information: update David Lee Wheeler · Nucleic Acids Research | 17 Dec 2003 | 11,002 |
| 17 | Current protocols in molecular biology Tom Sargent · Analytical Biochemistry | May 1988 | 7,304 |
| 18 | KEGG for linking genomes to life and the environment Minoru I. Kanehisa, Michihiro Araki, Susumu Goto et al. · Nucleic Acids Research | 12 Dec 2007 | 7,126 |
| 19 | Biopython: freely available Python tools for computational molecular biology and bioinformatics Peter Cock, Tiago Antão, Jeffrey T. Chang et al. · Bioinformatics | 20 Mar 2009 | 6,801 |
| 20 | Toward understanding the origin and evolution of cellular organisms Minoru I. Kanehisa · Protein Science | 23 Aug 2019 | 6,691 |
| 21 | LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions Andrew C. Wallace, Roman A. Laskowski, Janet M. Thornton · Protein Engineering Design and Selection | 1995 | 5,782 |
| 22 | CellProfiler: image analysis software for identifying and quantifying cell phenotypes Anne E. Carpenter, Thouis R. Jones, Michael R. Lamprecht et al. · Genome biology | 31 Oct 2006 | 5,571 |
| 23 | Understanding Molecular Simulation Daan Frenkel, Berend Smit, Jan Tobochnik et al. · Computers in Physics | Jul 1997 | 5,464 |
| 24 | COSMIC: the Catalogue Of Somatic Mutations In Cancer John G. Tate et al. · Nucleic Acids Research | 11 Oct 2018 | 5,379 |
| 25 | The Pfam protein families database in 2019 Sara El-Gebali, Jaina Mistry, Alex Bateman et al. · Nucleic Acids Research | 9 Oct 2018 | 5,256 |
| 26 | Cytoscape 2.8: new features for data integration and network visualization Michael E. Smoot, Keiichiro Ono, Johannes Ruscheinski et al. · Bioinformatics | 12 Dec 2010 | 5,078 |
| 27 | GSDS 2.0: an upgraded gene feature visualization server Bo Hu, Jinpu Jin, An‐Yuan Guo et al. · Bioinformatics | 10 Dec 2014 | 4,935 |
| 28 | The nf-core framework for community-curated bioinformatics pipelines Philip Ewels, Alexander Peltzer, Sven Fillinger et al. · Nature Biotechnology | 13 Feb 2020 | 4,389 |
| 29 | Systems Biology: A Brief Overview Hiroaki Kitano · Science | Mar 2002 | 4,305 |
| 30 | Small-Molecule Library Screening by Docking with PyRx Sargis Dallakyan, Arthur J. Olson · Methods in molecular biology | 22 Dec 2014 | 4,074 |
| 31 | Gene Action in the X-chromosome of the Mouse (Mus musculus L.) Mary Frances Lyon · Nature | Apr 1961 | 4,054 |
| 32 | The Galaxy platform for accessible, reproducible and collaborative biomedical analyses: 2018 update Enis Afgan, Dannon Baker, Bérénice Batut et al. · Nucleic Acids Research | 3 May 2018 | 4,017 |
| 33 | AliView: a fast and lightweight alignment viewer and editor for large datasets Anders O. Larsson · Bioinformatics | 5 Aug 2014 | 3,951 |
| 34 | Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation Ivica Letunić, Peer Bork · Bioinformatics | 18 Oct 2006 | 3,545 |
| 35 | High resolution two-dimensional electrophoresis of basic as well as acidic proteins Patricia Z. O’Farrell, Howard M. Goodman, Patrick H. O’Farrell · Cell | Dec 1977 | 3,541 |
| 36 | NanoPack: visualizing and processing long-read sequencing data Wouter De Coster, Svenn D’Hert, Darrin T. Schultz et al. · Bioinformatics | 12 Mar 2018 | 3,475 |
| 37 | PubChem 2019 update: improved access to chemical data Sunghwan Kim, Jie Chen, Tiejun Cheng et al. · Nucleic Acids Research | 26 Oct 2018 | 3,458 |
| 38 | VennDiagram: a package for the generation of highly-customizable Venn and Euler diagrams in R Hanbo Chen, Paul Christopher Boutros · BMC Bioinformatics | 26 Jan 2011 | 3,374 |
| 39 | Bandage: interactive visualization of de novo genome assemblies Ryan R. Wick, Mark B. Schultz, Justin Zobel et al. · Bioinformatics | 22 Jun 2015 | 3,373 |
| 40 | Unipro UGENE: a unified bioinformatics toolkit Konstantin Okonechnikov, Olga Golosova, Mikhail Fursov et al. · Bioinformatics | 24 Feb 2012 | 3,350 |
| 41 | Applications of machine learning in drug discovery and development Jessica J. Vamathevan, Dominic A. Clark, Paul Czodrowski et al. · Nature Reviews Drug Discovery | 11 Apr 2019 | 3,274 |
| 42 | Artemis: sequence visualization and annotation Kim Rutherford, Julian Parkhill, James Crook et al. · Bioinformatics | Oct 2000 | 3,127 |
| 43 | Multiple Sequence Alignment Using ClustalW and ClustalX Julie Thompson, Toby J. Gibson, Desmond G. Higgins · Current Protocols in Bioinformatics | Aug 2002 | 3,105 |
| 44 | Database resources of the national center for biotechnology information Eric W Sayers et al. · Nucleic Acids Research | 18 Nov 2021 | 3,075 |
| 45 | Applied Survival Analysis: Regression Modeling of Time-to-Event Data David W. Hosmer, Stanley A. Lemeshow, Susanne J. May | 7 Mar 2008 | 3,036 |
| 46 | The Significance of Digital Gene Expression Profiles Stéphane Audic, Jean‐Michel Claverie · Genome Research | Oct 1997 | 2,865 |
| 47 | A Completely Reimplemented MPI Bioinformatics Toolkit with a New HHpred Server at its Core Lukas Zimmermann, Andrew Wills Stephens, Seung‐Zin Nam et al. · Journal of Molecular Biology | 16 Dec 2017 | 2,850 |
| 48 | OMIM.org: Online Mendelian Inheritance in Man (OMIM®), an online catalog of human genes and genetic disorders Joanna S. Amberger, Carol A. Bocchini, François Schiettecatte et al. · Nucleic Acids Research | 26 Nov 2014 | 2,809 |
| 49 | Sambamba: fast processing of NGS alignment formats Artem Tarasov, Albert J. Vilella, Edwin P. J. G. Cuppen et al. · Bioinformatics | 19 Feb 2015 | 2,794 |
| 50 | The ImageJ ecosystem: An open platform for biomedical image analysis Johannes Schindelin, Curtis Rueden, Mark Hiner et al. · Molecular Reproduction and Development | Jul 2015 | 2,786 |
Citations are OpenAlex's own counts, from its 2026-09-23 snapshot, so the ranking can differ from Google Scholar or Web of Science. A paper is listed for a field when OpenAlex files it under one of the field's topics; retracted papers and papers whose citation count cannot be traced to citing works are left out, and a preprint and the article it became are shown once. Release dates are OpenAlex's: where a source gave only a month or a year, that is what is shown. How the lists are chosen.