| 1 |
The Cellular Thermal Shift Assay: A Novel Biophysical Assay for In Situ Drug Target Engagement and Mechanistic Biomarker Studies
Daniel Martinez Molina, Pär Nordlund · The Annual Review of Pharmacology and Toxicology
ReviewOpen access |
13 Nov 2015 |
313 |
| 2 |
Development of a Novel B-Cell Lymphoma 6 (BCL6) PROTAC To Provide Insight into Small Molecule Targeting of BCL6
William McCoull et al. · ACS Chemical Biology
Open access |
17 Oct 2018 |
141 |
| 3 |
ProTargetMiner as a proteome signature library of anticancer molecules for functional discovery
Amir Ata Saei, Christian Michel Beusch, Alexey L. Chernobrovkin et al. · Nature Communications
Open access |
16 Dec 2019 |
85 |
| 4 |
Cellularly active N-hydroxyurea FEN1 inhibitors block substrate entry to the active site
Jack C. Exell, Mark Jason Thompson, L. David Finger et al. · Nature Chemical Biology
Open access |
15 Aug 2016 |
79 |
| 5 |
Current Advances in CETSA
Tuomas Aleksi Tolvanen · Frontiers in Molecular Biosciences
Open access |
9 Jun 2022 |
75 |
| 6 |
Serum thymidine kinase 1 activity as a pharmacodynamic marker of cyclin-dependent kinase 4/6 inhibition in patients with early-stage breast cancer receiving neoadjuvant palbociclib
Nusayba Ali Bagegni, Shana N. Thomas, Ning Liu et al. · Breast Cancer Research
Open access |
21 Nov 2017 |
74 |
| 7 |
System-wide identification and prioritization of enzyme substrates by thermal analysis
Amir Ata Saei, Christian Michel Beusch, Pierre Sabatier et al. · Nature Communications
Open access |
26 Feb 2021 |
73 |
| 8 |
Selective release of miRNAs via extracellular vesicles is associated with house‐dust mite allergen‐induced airway inflammation
Yasuhiro Gon, S. Maruoka, Takuya Inoue et al. · Clinical & Experimental Allergy
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31 Aug 2017 |
67 |
| 9 |
Mass spectrometry-based Cellular Thermal Shift Assay (CETSA®) for target deconvolution in phenotypic drug discovery
Tomas Friman · Bioorganic & Medicinal Chemistry
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2 Nov 2019 |
66 |
| 10 |
Determining direct binders of the Androgen Receptor using a high-throughput Cellular Thermal Shift Assay
Joseph Shaw, Mathew Leveridge, Charlotta Norling et al. · Scientific Reports
Open access |
3 Jan 2018 |
59 |
| 11 |
Crystal structures of SAMHD1 inhibitor complexes reveal the mechanism of water-mediated dNTP hydrolysis
Elizabeth R. Morris, Sarah J. Caswell, Simone Kunzelmann et al. · Nature Communications
Open access |
23 Jun 2020 |
49 |
| 12 |
Positioning High-Throughput CETSA in Early Drug Discovery through Screening against B-Raf and PARP1
Joseph Shaw, Ian L. Dale, PAUL E. HEMSLEY et al. · SLAS DISCOVERY
Open access |
13 Dec 2018 |
49 |
| 13 |
CETSA: A Target Engagement Assay with Potential to Transform Drug Discovery
Annika Jenmalm Jensen, Daniel Martinez Molina, Thomas Lundbäck · Future Medicinal Chemistry
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Jun 2015 |
47 |
| 14 |
Cyclic 5-membered disulfides are not selective substrates of thioredoxin reductase, but are opened nonspecifically
Jan G. Felber, Lena Poczka, Karoline Scholzen et al. · Nature Communications
Open access |
Apr 2022 |
44 |
| 15 |
Proteome-wide cellular thermal shift assay reveals unexpected cross-talk between brassinosteroid and auxin signaling
Qing Lu, Yonghong Zhang, Joakim Hellner et al. · Proceedings of the National Academy of Sciences
Open access |
7 Mar 2022 |
37 |
| 16 |
A Tale of Two Tails: Efficient Profiling of Protein Degraders by Specific Functional and Target Engagement Readouts
Alexey L. Chernobrovkin, Cindy Cázares-Körner, Tomas Friman et al. · SLAS DISCOVERY
Open access |
15 Jan 2021 |
31 |
| 17 |
Discovery and Characterization of the Potent and Highly Selective 1,7-Naphthyridine-Based Inhibitors BAY-091 and BAY-297 of the Kinase PIP4K2A
Lars Wortmann et al. · Journal of Medicinal Chemistry
Open access |
26 Oct 2021 |
27 |
| 18 |
Abnormal (Hydroxy)proline Deuterium Content Redefines Hydrogen Chemical Mass
Hassan Gharibi, Alexey L. Chernobrovkin, Gunilla M. Eriksson et al. · Journal of the American Chemical Society
Open access |
2 Feb 2022 |
25 |
| 19 |
CETSA MS Profiling for a Comparative Assessment of FDA-Approved Antivirals Repurposed for COVID-19 Therapy Identifies TRIP13 as a Remdesivir Off-Target
Tomas Friman, Alexey L. Chernobrovkin, Daniel Martinez Molina et al. · SLAS DISCOVERY
Open access |
19 Nov 2020 |
24 |
| 20 |
Generation and validation of recombinant antibodies to study human aminoacyl-tRNA synthetases
Charlotta Preger, Edvard Wigren, Elena Ossipova et al. · Journal of Biological Chemistry
Open access |
14 Aug 2020 |
22 |
| 21 |
On the Relationship between Molecular Hit Rates in High-Throughput Screening and Molecular Descriptors
Mari Hansson, John Stuart Pemberton, Ola Engkvist et al. · SLAS DISCOVERY
Open access |
24 Aug 2013 |
20 |
| 22 |
Deciphering the Allosteric Binding Mechanism of the Human Tropomyosin Receptor Kinase A (hTrkA) Inhibitors
Govindan Subramanian, Paul D. Johnson, Theresa Zachary et al. · ACS Chemical Biology
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6 May 2019 |
17 |
| 23 |
Proteomics-Compatible Fourier Transform Isotopic Ratio Mass Spectrometry of Polypeptides
Hassan Gharibi, Alexey L. Chernobrovkin, Amir Ata Saei et al. · Analytical Chemistry
Open access |
17 Oct 2022 |
16 |
| 24 |
A Shift in Thinking: Cellular Thermal Shift Assay-Enabled Drug Discovery
Isabel Martín Caballero, Stina Lundgren · ACS Medicinal Chemistry Letters
Open access |
17 Mar 2023 |
15 |
| 25 |
High throughput screening of 0.5 million compounds against CRAF using Alpha CETSAⓇ
Hannah Rowlands, Kirsten Tschapalda, Carolyn Blackett et al. · SLAS DISCOVERY
Open access |
Feb 2023 |
15 |
| 26 |
Mechanistic Insights into a CDK9 Inhibitor Via Orthogonal Proteomics Methods
J. Adam Hendricks et al. · ACS Chemical Biology
Open access |
25 Dec 2021 |
14 |
| 27 |
Inverse agonism of lysophospholipids with cationic head groups at Gi-coupled receptor GPR82
Daisuke Yasuda, Fumie Hamano, Kazuyuki Masuda et al. · European Journal of Pharmacology
Open access |
29 Jun 2023 |
10 |
| 28 |
In-depth characterization of Staurosporine induced proteome thermal stability changes
Alexey L. Chernobrovkin, Johan Lengqvist, Cindy Körner et al. · bioRxiv (Cold Spring Harbor Laboratory)
PreprintOpen access |
15 Mar 2020 |
10 |
| 29 |
Positive Modulation of the Glycine Receptor by Means of Glycine Receptor–Binding Aptamers
Nancy Dekki Shalaly, Eduardo Aneiros, Michael Blank et al. · SLAS DISCOVERY
Open access |
12 Jun 2015 |
10 |
| 30 |
Discovery of 3-Amino-1H-pyrazole-Based Kinase Inhibitors to Illuminate the Understudied PCTAIRE Family
Jennifer Alisa Amrhein, Lena M. Berger, Amelie Tjaden et al. · International Journal of Molecular Sciences
Open access |
27 Nov 2022 |
9 |
| 31 |
Identification and evaluation of small-molecule inhibitors against the dNTPase SAMHD1 via a comprehensive screening funnel
Si Min Zhang et al. · iScience
Open access |
19 Jan 2024 |
8 |
| 32 |
Extending in Silico Protein Target Prediction Models to Include Functional Effects
Lewis Mervin, Avid M. Afzal, Lars Brive et al. · Frontiers in Pharmacology
Open access |
11 Jun 2018 |
8 |
| 33 |
System-wide identification and prioritization of enzyme substrates by thermal analysis (SIESTA)
Amir Ata Saei, Christian M. Beusch, Pierre Sabatier et al. · bioRxiv (Cold Spring Harbor Laboratory)
PreprintOpen access |
21 Sep 2018 |
6 |
| 34 |
Neuroprotective and Disease Modifying Effects of the Triazinetrione ACD856, a Positive Allosteric Modulator of Trk-Receptors for the Treatment of Cognitive Dysfunction in Alzheimer’s Disease
Cristina Parrado‐Fernández, Sanja Juric, Maria Backlund et al. · Preprints.org
PreprintOpen access |
8 Jun 2023 |
5 |
| 35 |
Development of pyrazolo[1,5-a]pyrimidine based macrocyclic kinase inhibitors targeting AAK1
Theresa Elisabeth Mensing, Christian Georg Kurz, Jennifer Alisa Amrhein et al. · European Journal of Medicinal Chemistry
Open access |
22 Aug 2025 |
4 |
| 36 |
ProTargetMiner: A proteome signature library of anticancer molecules for functional discovery
Amir Ata Saei, Alexey L. Chernobrovkin, Pierre Sabatier et al. · bioRxiv (Cold Spring Harbor Laboratory)
PreprintOpen access |
18 Sep 2018 |
4 |
| 37 |
Phenomics‐Based Discovery of Novel Orthosteric Choline Kinase Inhibitors
Ludwig G. Bauer et al. · Angewandte Chemie International Edition
Open access |
30 Dec 2024 |
2 |
| 38 |
Discovery of RNA-Protein Molecular Clamps Using Proteome-Wide Stability Assays
Stanley I. Goldstein, Alice C. Fan, Zihao Wang et al. · bioRxiv (Cold Spring Harbor Laboratory)
PreprintOpen access |
19 Apr 2024 |
2 |
| 39 |
A mass spectrometry‐based non‐radioactive differential radial capillary action of ligand assay (DRaCALA) to assess ligand binding to proteins
Annika Cimdins, Alexey L. Chernobrovkin, Sookyoung Kim et al. · Journal of Mass Spectrometry
Open access |
14 Mar 2022 |
2 |
| 40 |
Multivariate mining of an alpaca immune repertoire identifies potent cross-neutralising SARS-CoV-2 nanobodies
Leo Hanke, Daniel J. Sheward, Alec P. Pankow et al. · bioRxiv (Cold Spring Harbor Laboratory)
PreprintOpen access |
26 Jul 2021 |
2 |
| 41 |
SIESTA as a universal unbiased proteomics approach for identification and prioritization of enzyme substrates
Amir Ata Saei, Christian Michel Beusch, Pierre Sabatier et al. · Research Square
PreprintOpen access |
26 Feb 2021 |
2 |
| 42 |
Quantitative target engagement of RIPK1 in human whole blood via the cellular thermal shift assay for potential pre-clinical and clinical applications
Shitalben Patel, Marie Karlsson, Joseph T. Klahn et al. · SLAS DISCOVERY
Open access |
13 Dec 2023 |
1 |
| 43 |
Morphological and Mechanistic Aspects of Thiourea-Induced Acute Lung Injury and Tolerance in the Rat
Giovanni Pellegrini, Dominic P. Williams, Daniele Amadio et al. · Toxicologic Pathology
Open access |
Aug 2020 |
1 |
| 44 |
Toward a Species Search Engine: KISSE Offers a Rigorous Statistical Framework for Bone Collagen Tandem Mass Spectrometry Data
Hassan Gharibi, Amir Ata Saei, Alexey L. Chernobrovkin et al. · Advanced Science
Open access |
11 Aug 2025 |
0 |
| 45 |
Role of Structural Modifications in Peptidomimetic Compounds as Potential Antimicrobial Agents against Staphylococcus aureus and Streptococcus pyogenes: Balancing Bioavailability, Safety, and Antimicrobial Activity
Maria Dzierżyńska, Justyna Sawicka, Katarzyna Łada et al. · ACS Omega
Open access |
22 Jul 2025 |
0 |
| 46 |
CETSA-MS unveils novel targets engaged by rigosertib to promote anti-tumor activity and inflammatory responses
Petros Kechagioglou, Hajime Yurugi, Camille Amandine Dupont et al. · iScience
Open access |
26 May 2025 |
0 |
| 47 |
Towards a species search engine: KISSE offers a rigorous statistical framework for bone collagen tandem mass spectrometry data
Hassan Gharibi, Amir Ata Saei, Alexey L. Chernobrovkin et al. · bioRxiv (Cold Spring Harbor Laboratory)
PreprintOpen access |
26 Mar 2025 |
0 |
| 48 |
Phänomik‐basierte Entdeckung neuer orthosterischer Cholinkinase Inhibitoren
Ludwig G. Bauer et al. · Angewandte Chemie
Open access |
30 Dec 2024 |
0 |
| 49 |
Exploring the Limits of High-Density Perimetry for Characterizing Mild Defects
Iván Marín‐Franch, Harry J. Wyatt, William H. Swanson · SSRN Electronic Journal
PreprintOpen access |
2022 |
0 |
| 50 |
Proteome-wide cellular thermal shift assay reveals novel crosstalk between brassinosteroid and auxin signaling
Qing Lu, Yonghong Zhang, Joakim Hellner et al. · bioRxiv (Cold Spring Harbor Laboratory)
PreprintOpen access |
16 Dec 2021 |
0 |