Most cited papers in Biochemical and Molecular Research
What are the most cited papers in Biochemical and Molecular Research?
The most cited paper in Biochemical and Molecular Research is "Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix" (Dorothee Claudia Liebschner et al., 2019), with 7,679 citations. Next come "Genetic regulatory mechanisms in the synthesis of proteins" (6,659) and "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase" (5,982).
Showing 50 of 100 papers. Every paper with more than 923 citations is listed.
| # | Paper | ||
|---|---|---|---|
| 1 | Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix Dorothee Claudia Liebschner et al. · Acta Crystallographica Section D Structural Biology | Oct 2019 | 7,679 |
| 2 | Genetic regulatory mechanisms in the synthesis of proteins François Jacob, Jacques Monod · Journal of Molecular Biology | Jun 1961 | 6,659 |
| 3 | Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Donald B. Smith, Kevin Scot Johnson · Gene | Jul 1988 | 5,982 |
| 4 | A Protein Binding Assay for Adenosine 3′:5′-Cyclic Monophosphate Alfred G. Gilman · Proceedings of the National Academy of Sciences | Sep 1970 | 3,270 |
| 5 | The growing impact of click chemistry on drug discovery Hartmuth Christian Kolb, Karl Barry Sharpless · Drug Discovery Today | Dec 2003 | 3,149 |
| 6 | Automated MAD and MIR structure solution Thomas C. Terwilliger, Joel Berendzen · Acta Crystallographica Section D Biological Crystallography | Apr 1999 | 3,043 |
| 7 | Identification of the von Hippel-Lindau Disease Tumor Suppressor Gene Farida Latif et al. · Science | 28 May 1993 | 3,023 |
| 8 | Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues. F Thiébaut, Takashi Tsuruo, Hironobu Hamada et al. · Proceedings of the National Academy of Sciences | Nov 1987 | 2,807 |
| 9 | Cellular uptake of the tat protein from human immunodeficiency virus Alan D. Frankel, Carl O. Pabo · Cell | Dec 1988 | 2,798 |
| 10 | DNA Topoisomerases: Structure, Function, and Mechanism James J. Champoux · Annual Review of Biochemistry | Jun 2001 | 2,754 |
| 11 | Transduction of linked genetic characters of the host by bacteriophage P1 E. S. Lennox · Virology | Jul 1955 | 2,612 |
| 12 | K-Ras(G12C) inhibitors allosterically control GTP affinity and effector interactions Jonathan M. L. Ostrem, Ulf Peters, Martin L. Sos et al. · Nature | Nov 2013 | 2,587 |
| 13 | STRUCTURE AND FUNCTIONS OF THE 20S AND 26S PROTEASOMES Olivier Coux, Keiji Tanaka, Alfred L. Goldberg · Annual Review of Biochemistry | Jun 1996 | 2,447 |
| 14 | Starch gel electrophoresis of enzymes?A compilation of recipes Charles R. Shaw, Rupi Prasad · Biochemical Genetics | Apr 1970 | 2,441 |
| 15 | Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product Yoshizumi Ishino, Hideo Shinagawa, K Makino et al. · Journal of Bacteriology | Dec 1987 | 2,405 |
| 16 | Exploiting the PI3K/AKT Pathway for Cancer Drug Discovery Bryan T. Hennessy, Debra L. Smith, Prahlad T. Ram et al. · Nature Reviews Drug Discovery | Dec 2005 | 2,188 |
| 17 | Data processing and analysis with the autoPROC toolbox Clemens Vonrhein, Claus Flensburg, Peter M. Keller et al. · Acta Crystallographica Section D Biological Crystallography | 17 Mar 2011 | 2,177 |
| 18 | The mechanism of action of cyclosporin A and FK506 Stuart L. Schreiber, Gerald R Crabtree · Immunology Today | 1992 | 2,153 |
| 19 | Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability Claire Vieille, G. Zeikus · Microbiology and Molecular Biology Reviews | Mar 2001 | 2,140 |
| 20 | Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction Boudewijn M.T. Burgering, Paul James Coffer · Nature | Aug 1995 | 2,112 |
| 21 | Hybrid restriction enzymes: zinc finger fusions to Fok I cleavage domain. Y G Kim, Jihye Cha, Srinivasan Chandrasegaran · Proceedings of the National Academy of Sciences | 6 Feb 1996 | 2,101 |
| 22 | The kinetics of enzyme-catalyzed reactions with two or more substrates or products☆I. Nomenclature and rate equations William W. Cleland · Biochimica et Biophysica Acta | 1963 | 2,100 |
| 23 | Discovering High-Affinity Ligands for Proteins: SAR by NMR Suzanne Beckham Shuker, Philip J. Hajduk, Robert P. Meadows et al. · Science | 29 Nov 1996 | 2,061 |
| 24 | The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase Thomas F. Franke, Sung‐Il Yang, Tung O. Chan et al. · Cell | Jun 1995 | 2,033 |
| 25 | Atomic structure of the actin: DNase I complex Wolfgang Kabsch, Hans Georg Mannherz, Dietrich Suck et al. · Nature | Sep 1990 | 1,944 |
| 26 | Physiological concentrations of purines and pyrimidines Thomas W. Traut · Molecular and Cellular Biochemistry | 1994 | 1,917 |
| 27 | The kinetics of enzyme-catalyzed reactions with two or more substrates or products William W. Cleland · Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects | 1963 | 1,911 |
| 28 | Structure of ubiquitin refined at 1.8 Å resolution Senadhi Vijay‐Kumar, Charles E. Bugg, William J. Cook · Journal of Molecular Biology | Apr 1987 | 1,793 |
| 29 | Crystal Structure of the Catalytic Subunit of Cyclic Adenosine Monophosphate-Dependent Protein Kinase Daniel R. Knighton, Jianhua Zheng, Lynn F. Ten Eyck et al. · Science | 26 Jul 1991 | 1,781 |
| 30 | Small-molecule inhibitors of protein–protein interactions: progressing towards the dream Michelle R. Arkin, James A. Wells · Nature Reviews Drug Discovery | Apr 2004 | 1,713 |
| 31 | The protein kinase B/Akt signalling pathway in human malignancy K. M. Nicholson, Neil G. Anderson · Cellular Signalling | May 2002 | 1,649 |
| 32 | A Sequencing Method Based on Real-Time Pyrophosphate Mostafa Ronaghi, Mathias Uhlén, Pål Nyrén · Science | 17 Jul 1998 | 1,631 |
| 33 | Prodrugs: design and clinical applications Jarkko Rautio, Hanna Kumpulainen, Tycho Heimbach et al. · Nature Reviews Drug Discovery | 25 Jan 2008 | 1,626 |
| 34 | Bacterial alkaline proteases: molecular approaches and industrial applications Rani Gupta, Beg Q., Philipp Lorenz · Applied Microbiology and Biotechnology | Jun 2002 | 1,611 |
| 35 | Selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl)guanine Gertrude B. Elion, Phillip A. Furman, James A. Fyfe et al. · Proceedings of the National Academy of Sciences | Dec 1977 | 1,595 |
| 36 | Serine/Threonine Phosphatases: Mechanism through Structure Yigong Shi · Cell | Oct 2009 | 1,527 |
| 37 | Tumor glycolysis as a target for cancer therapy: progress and prospects Shanmugasundaram Ganapathy‐Kanniappan, Jean-François H. Geschwind · Molecular Cancer | 2013 | 1,490 |
| 38 | A familial disorder of uric acid metabolism and central nervous system function Michael Lesch, William L. Nyhan · The American Journal of Medicine | Apr 1964 | 1,468 |
| 39 | Approved Antiviral Drugs over the Past 50 Years Erik De Clercq, Guangdi Li · Clinical Microbiology Reviews | 9 Jun 2016 | 1,465 |
| 40 | An Adenosine 3′,5′-Monophosphate-dependant Protein Kinase from Rabbit Skeletal Muscle Donal A. Walsh, John P. Perkins, E G Krebs · Journal of Biological Chemistry | Jul 1968 | 1,453 |
| 41 | Hepatic Triphosphopyridine Nucleotide-Cytochrome c Reductase: Isolation, Characterization, and Kinetic Studies Alvah H. Phillips, ROBERT G. LANGDON · Journal of Biological Chemistry | Aug 1962 | 1,433 |
| 42 | Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling Joanna C. Betts, Pauline T. Lukey, Linda C. Robb et al. · Molecular Microbiology | Feb 2002 | 1,426 |
| 43 | Efficient Initiation of HCV RNA Replication in Cell Culture Keril J. Blight, Alexander A. Kolykhalov, Charles M. Rice · Science | 8 Dec 2000 | 1,416 |
| 44 | The Genome Sequence of Trypanosoma cruzi , Etiologic Agent of Chagas Disease Najib M. El-Sayed et al. · Science | 14 Jul 2005 | 1,412 |
| 45 | inhA , a Gene Encoding a Target for Isoniazid and Ethionamide in Mycobacterium tuberculosis Asesh Banerjee, Eugenie Dubnau, Annaı̈k Quémard et al. · Science | 14 Jan 1994 | 1,405 |
| 46 | Regulation of uric acid metabolism and excretion Jessica Maiuolo, Francesca Oppedisano, Santo Gratteri et al. · International Journal of Cardiology | 14 Aug 2015 | 1,404 |
| 47 | Calcineurin: Form and Function Frank M. Rusnak, Pamela S. Mertz · Physiological Reviews | 10 Jan 2000 | 1,389 |
| 48 | Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region Peter Simmonds, Bruce D. Irvine, P.L. Yap et al. · Journal of General Virology | Nov 1993 | 1,385 |
| 49 | Phosphorylation of 3'-azido-3'-deoxythymidine and selective interaction of the 5'-triphosphate with human immunodeficiency virus reverse transcriptase. P A Furman, James A. Fyfe, M H St Clair et al. · Proceedings of the National Academy of Sciences | Nov 1986 | 1,356 |
| 50 | DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells. Michael H. Wigler, Àngel Pellicer, Saul J. Silverstein et al. · Proceedings of the National Academy of Sciences | Mar 1979 | 1,340 |
Citations are OpenAlex's own counts, from its 2026-09-23 snapshot, so the ranking can differ from Google Scholar or Web of Science. A paper is listed for a field when OpenAlex files it under one of the field's topics; retracted papers and papers whose citation count cannot be traced to citing works are left out, and a preprint and the article it became are shown once. Release dates are OpenAlex's: where a source gave only a month or a year, that is what is shown. How the lists are chosen.