Topic · CRISPR and Genetic Engineering
Most cited papers in CRISPR and Genetic Engineering
What are the most cited papers in CRISPR and Genetic Engineering?
The most cited paper in CRISPR and Genetic Engineering is "Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors" (Kazutoshi Takahashi, Shinya Yamanaka, 2006), with 26,957 citations. Next come "A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity" (17,763) and "Multiplex Genome Engineering Using CRISPR/Cas Systems" (16,026).
Showing 50 of 100 papers. Every paper with more than 3,023 citations is listed.
| # | Paper | ||
|---|---|---|---|
| 1 | Induction of Pluripotent Stem Cells from Mouse Embryonic and Adult Fibroblast Cultures by Defined Factors Kazutoshi Takahashi, Shinya Yamanaka · Cell | Aug 2006 | 26,957 |
| 2 | A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity Martin Jínek, Krzysztof Chylinski, Ines Fonfara et al. · Science | 29 Jun 2012 | 17,763 |
| 3 | Multiplex Genome Engineering Using CRISPR/Cas Systems Le Dinh Công, F. Ann Ran, David B. T. Cox et al. · Science | 4 Jan 2013 | 16,026 |
| 4 | Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans Andrew Z. Fire, Siqun Xu, Mary Katherine Montgomery et al. · Nature | Feb 1998 | 15,508 |
| 5 | One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences | 30 May 2000 | 15,367 |
| 6 | The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14 Rosalind C. Lee, Rhonda L. Feinbaum, Victor Ambros · Cell | Dec 1993 | 13,024 |
| 7 | Enrichr: a comprehensive gene set enrichment analysis web server 2016 update Maxim V. Kuleshov, Matthew R. Jones, Andrew D. Rouillard et al. · Nucleic Acids Research | 3 May 2016 | 12,102 |
| 8 | Genome engineering using the CRISPR-Cas9 system F. Ann Ran, Patrick D. Hsu, Jason B. Wright et al. · Nature Protocols | 24 Oct 2013 | 11,982 |
| 9 | Enzymatic assembly of DNA molecules up to several hundred kilobases Daniel G. Gibson, Lei Young, Ray-Yuan Chuang et al. · Nature Methods | 12 Apr 2009 | 11,139 |
| 10 | The mutational constraint spectrum quantified from variation in 141,456 humans Konrad J. Karczewski et al. · Nature | 27 May 2020 | 10,422 |
| 11 | Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells Junying Yu, Maxim A. Vodyanik, Kim Smuga-Otto et al. · Science | 20 Nov 2007 | 10,385 |
| 12 | RNA-Guided Human Genome Engineering via Cas9 Prashant Mali, Luhan Yang, Kevin M. Esvelt et al. · Science | 4 Jan 2013 | 9,548 |
| 13 | Loop-mediated isothermal amplification of DNA Tsugunori Notomi · Nucleic Acids Research | 15 Jun 2000 | 8,938 |
| 14 | Establishment in culture of pluripotential cells from mouse embryos Martin John Evans, M. H. Kaufman · Nature | Jul 1981 | 8,657 |
| 15 | Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection Tomoya Baba, Takeshi Ara, Miki Launois Hasegawa et al. · Molecular Systems Biology | 21 Feb 2006 | 8,362 |
| 16 | Site-directed mutagenesis by overlap extension using the polymerase chain reaction Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene | Apr 1989 | 7,540 |
| 17 | A Broad Host Range Mobilization System for In Vivo Genetic Engineering: Transposon Mutagenesis in Gram Negative Bacteria Reinhard Simon, U. B. Priefer, Alfred Pühler · Nature Biotechnology | Nov 1983 | 7,350 |
| 18 | The new frontier of genome engineering with CRISPR-Cas9 Jennifer A. Doudna, Emmanuelle Charpentier · Science | 28 Nov 2014 | 7,176 |
| 19 | MicroRNAs: small RNAs with a big role in gene regulation Lin He, Gregory James Hannon · Nature Reviews Genetics | 21 Jun 2004 | 7,124 |
| 20 | Global quantification of mammalian gene expression control Björn Schwanhäußer, Dorothea Busse, Na Li et al. · Nature | 17 May 2011 | 7,057 |
| 21 | Green Fluorescent Protein as a Marker for Gene Expression Martin Chalfie, Yuan Tu, Ghia M. Euskirchen et al. · Science | 11 Feb 1994 | 6,824 |
| 22 | Targeting the DNA repair defect in BRCA mutant cells as a therapeutic strategy Hannah Farmer, Nuala McCabe, Christopher J. Lord et al. · Nature | Apr 2005 | 6,785 |
| 23 | CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes Rodolphe Barrangou, Christophe Fremaux, Hélène Deveau et al. · Science | 22 Mar 2007 | 6,479 |
| 24 | Phosphorylation and Regulation of Akt/PKB by the Rictor-mTOR Complex Dos D. Sarbassov, David A. Guertin, Siraj Mahamed Ali et al. · Science | 17 Feb 2005 | 6,276 |
| 25 | The DNA-damage response in human biology and disease Stephen P. Jackson, Jiří Bártek · Nature | 20 Oct 2009 | 6,194 |
| 26 | A Strong Candidate for the Breast and Ovarian Cancer Susceptibility Gene BRCA1 Yoshio Miki et al. · Science | 7 Oct 1994 | 6,134 |
| 27 | Development and Applications of CRISPR-Cas9 for Genome Engineering Patrick D. Hsu, Eric S. Lander, Feng Zhang · Cell | Jun 2014 | 5,993 |
| 28 | Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage Alexis C. Komor, Y. Bill Kim, Michael S. Packer et al. · Nature | 19 Apr 2016 | 5,677 |
| 29 | Improved vectors and genome-wide libraries for CRISPR screening Neville E. Sanjana, Ophir Shalem, Feng Zhang · Nature Methods | 30 Jul 2014 | 5,675 |
| 30 | Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells Ophir Shalem, Neville E. Sanjana, Ella Hartenian et al. · Science | 12 Dec 2013 | 5,627 |
| 31 | High-efficiency transformation of mammalian cells by plasmid DNA. C Chen, Hiroto Okayama · Molecular and Cellular Biology | Aug 1987 | 5,335 |
| 32 | Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression Lei Stanley Qi, Matthew H. Larson, Luke A. Gilbert et al. · Cell | Feb 2013 | 5,298 |
| 33 | Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase Helen E. Bryant, Niklas Schultz, Huw D. Thomas et al. · Nature | 13 Apr 2005 | 5,263 |
| 34 | Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 John G. Doench, Nicolò Fusi, Meagan E. Sullender et al. · Nature Biotechnology | 18 Jan 2016 | 5,165 |
| 35 | The nuclear RNase III Drosha initiates microRNA processing Yoontae Lee, Chiyoung Ahn, Jinju Han et al. · Nature | Sep 2003 | 5,144 |
| 36 | Genome-Wide Insertional Mutagenesis of Arabidopsis thaliana José M. Alonso et al. · Science | 31 Jul 2003 | 5,137 |
| 37 | Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System Bernd Zetsche, Jonathan S. Gootenberg, Omar O. Abudayyeh et al. · Cell | 26 Sep 2015 | 5,035 |
| 38 | DNA targeting specificity of RNA-guided Cas9 nucleases Patrick D. Hsu, David A. Scott, Joshua A. Weinstein et al. · Nature Biotechnology | 21 Jul 2013 | 5,032 |
| 39 | The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans Brenda J. Reinhart, Frank John Slack, Michael Basson et al. · Nature | Feb 2000 | 5,025 |
| 40 | Identification of Novel Genes Coding for Small Expressed RNAs Mariana Lagos‐Quintana, Reinhard Rauhut, Winfried Lendeckel et al. · Science | 26 Oct 2001 | 4,985 |
| 41 | In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector Luigi Naldini, Ulrike Blömer, Philippe A. Gallay et al. · Science | 12 Apr 1996 | 4,952 |
| 42 | Role for a bidentate ribonuclease in the initiation step of RNA interference Emily Bernstein, Amy A. Caudy, Scott M. Hammond et al. · Nature | 2001 | 4,947 |
| 43 | Search-and-replace genome editing without double-strand breaks or donor DNA Andrew V. Anzalone, Peyton B. Randolph, Jessie R. Davis et al. · Nature | 21 Oct 2019 | 4,928 |
| 44 | KEGG: integrating viruses and cellular organisms Minoru I. Kanehisa, Miho Furumichi, Yoko Sato et al. · Nucleic Acids Research | 9 Oct 2020 | 4,784 |
| 45 | A Simple and General Method for Transferring Genes into Plants R. Horsch, Joyce E. Fry, Nancy L. Hoffmann et al. · Science | 8 Mar 1985 | 4,784 |
| 46 | Full-length RNA-seq from single cells using Smart-seq2 Simone Picelli, Omid R. Faridani, Åsa K. Björklund et al. · Nature Protocols | 2014 | 4,639 |
| 47 | CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity Janice S. Chen, Enbo Ma, Lucas B. Harrington et al. · Science | 15 Feb 2018 | 4,623 |
| 48 | Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells Laurie A. Boyer, Tong Ihn Lee, Megan F. Cole et al. · Cell | Sep 2005 | 4,557 |
| 49 | Master Transcription Factors and Mediator Establish Super-Enhancers at Key Cell Identity Genes Warren A. Whyte, David A. Orlando, Denes Hnisz et al. · Cell | Apr 2013 | 4,469 |
| 50 | The DNA Damage Response: Making It Safe to Play with Knives Alberto Ciccia, Stephen J. Elledge · Molecular Cell | Oct 2010 | 4,446 |
Citations are OpenAlex's own counts, from its 2026-09-23 snapshot, so the ranking can differ from Google Scholar or Web of Science. A paper is listed for a field when OpenAlex files it under one of the field's topics; retracted papers and papers whose citation count cannot be traced to citing works are left out, and a preprint and the article it became are shown once. Release dates are OpenAlex's: where a source gave only a month or a year, that is what is shown. How the lists are chosen.