Most cited papers in Mass Spectrometry Techniques and Applications
What are the most cited papers in Mass Spectrometry Techniques and Applications?
The most cited paper in Mass Spectrometry Techniques and Applications is "Detection of specific sequences among DNA fragments separated by gel electrophoresis" (E.M. Southern, 1975), with 32,849 citations. Next come "GROMACS 4: Algorithms for Highly Efficient, Load-Balanced, and Scalable Molecular Simulation" (16,214) and "MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification" (15,998).
Showing 50 of 100 papers. Every paper with more than 2,321 citations is listed.
| # | Paper | ||
|---|---|---|---|
| 1 | Detection of specific sequences among DNA fragments separated by gel electrophoresis E.M. Southern · Journal of Molecular Biology | Nov 1975 | 32,849 |
| 2 | GROMACS 4: Algorithms for Highly Efficient, Load-Balanced, and Scalable Molecular Simulation Berk Hess, Carsten Kutzner, David van der Spoel et al. · Journal of Chemical Theory and Computation | 2 Feb 2008 | 16,214 |
| 3 | MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification Jürgen Cox, Matthias Mann · Nature Biotechnology | 23 Nov 2008 | 15,998 |
| 4 | CHARMM : A program for macromolecular energy, minimization, and dynamics calculations Bernard R. Brooks, Robert E. Bruccoleri, Barry D. Olafson et al. · Journal of Computational Chemistry | Jun 1983 | 15,019 |
| 5 | A low-viscosity epoxy resin embedding medium for electron microscopy Arthur R. Spurr · Journal of Ultrastructure Research | 1969 | 12,956 |
| 6 | Improved methods for building protein models in electron density maps and the location of errors in these models T. Alwyn Jones, Jin-yu Zou, Sandra W. Cowan et al. · Acta Crystallographica Section A Foundations of Crystallography | Mar 1991 | 12,692 |
| 7 | Deviations from michaelis-menten behaviour of plant glutamate dehydrogenase with ammonium as variable substrate Edwin Pahlich, Chr. Gerlitz · Phytochemistry | 1980 | 10,703 |
| 8 | CHARMM‐GUI: A web‐based graphical user interface for CHARMM Sunhwan Jo, Taehoon Kim, Vidyashankara Iyer et al. · Journal of Computational Chemistry | 19 Mar 2008 | 10,125 |
| 9 | Enzymatic Determination of Total Serum Cholesterol Charles C. Allain, Lucy S Poon, Cicely S G Chan et al. · Clinical Chemistry | Apr 1974 | 9,199 |
| 10 | Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels Andrej Shevchenko, Matthias S. Wilm, Ole Vorm et al. · Analytical Chemistry | Mar 1996 | 9,120 |
| 11 | Protein Identification and Analysis Tools on the ExPASy Server Elisabeth Gasteiger, Christine Hoogland, Alexandre Gattiker et al. · Humana Press eBooks | 2005 | 8,916 |
| 12 | Universal sample preparation method for proteome analysis Jacek R. Wiśniewski, Alexandre Zougman, Nagarjuna Nagaraj et al. · Nature Methods | 19 Apr 2009 | 8,878 |
| 13 | REFMAC 5 for the refinement of macromolecular crystal structures Garib N. Murshudov, Pavol Skubák, Andrey Andreyevich Lebedev et al. · Acta Crystallographica Section D Biological Crystallography | 17 Mar 2011 | 8,771 |
| 14 | A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels William M. Bonner, Ronald Alfred Laskey · European Journal of Biochemistry | Jul 1974 | 8,651 |
| 15 | A simplification of the protein assay method of Lowry et al. which is more generally applicable Gary L. Peterson · Analytical Biochemistry | Dec 1977 | 8,575 |
| 16 | Probability-based protein identification by searching sequence databases using mass spectrometry data David N. Perkins, Darryl Pappin, David M. Creasy et al. · Electrophoresis | Dec 1999 | 8,335 |
| 17 | PTRAJ and CPPTRAJ: Software for Processing and Analysis of Molecular Dynamics Trajectory Data Daniel R. Roe, Thomas E. Cheatham · Journal of Chemical Theory and Computation | 10 Jun 2013 | 7,841 |
| 18 | Electrospray Ionization for Mass Spectrometry of Large Biomolecules John B. Fenn, Matthias Mann, Chin Kai Meng et al. · Science | 6 Oct 1989 | 7,638 |
| 19 | Plasma perspective on strong field multiphoton ionization P. B. Corkum · Physical Review Letters | 27 Sep 1993 | 7,217 |
| 20 | GROMACS 3.0: a package for molecular simulation and trajectory analysis Erik R. Lindahl, Berk Hess, David van der Spoel · Journal of Molecular Modeling | Aug 2001 | 7,048 |
| 21 | Mass spectrometry-based proteomics Ruedi Aebersold, Matthias Mann · Nature | Mar 2003 | 6,961 |
| 22 | The Structure of the Potassium Channel: Molecular Basis of K + Conduction and Selectivity Declan Anthony Doyle, João Henrique Morais-Cabral, Richard A. Pfuetzner et al. · Science | 3 Apr 1998 | 6,890 |
| 23 | The PRIDE database resources in 2022: a hub for mass spectrometry-based proteomics evidences Yasset Pérez‐Riverol, Jingwen Bai, Chakradhar Bandla et al. · Nucleic Acids Research | 15 Oct 2021 | 6,874 |
| 24 | An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database Jimmy K. Eng, Ashley L. McCormack, John R. Yates · Journal of the American Society for Mass Spectrometry | Nov 1994 | 6,681 |
| 25 | Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride–methanol William R. Morrison, Lloyd M. Smith · Journal of Lipid Research | Oct 1964 | 6,001 |
| 26 | Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics Shao‐En Ong, Blagoy Blagoev, Irina Kratchmarova et al. · Molecular & Cellular Proteomics | May 2002 | 5,654 |
| 27 | A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures Robert G. Martin, Bruce N. Ames · Journal of Biological Chemistry | May 1961 | 5,579 |
| 28 | Determination of protein: A modification of the lowry method that gives a linear photometric response Edward F. Hartree · Analytical Biochemistry | Aug 1972 | 5,552 |
| 29 | XCMS: Processing Mass Spectrometry Data for Metabolite Profiling Using Nonlinear Peak Alignment, Matching, and Identification Colin A. Smith, Elizabeth J. Want, Grace O’Maille et al. · Analytical Chemistry | 7 Jan 2006 | 5,419 |
| 30 | The MaxQuant computational platform for mass spectrometry-based shotgun proteomics Stefka Tyanova, Tikira Temu, Jüergen Cox · Nature Protocols | 27 Oct 2016 | 5,351 |
| 31 | Strategies for the Assessment of Matrix Effect in Quantitative Bioanalytical Methods Based on HPLC−MS/MS Bogdan K. Matuszewski, Marvin L. Constanzer, C.M. Chavez-Eng · Analytical Chemistry | 4 Jun 2003 | 5,337 |
| 32 | In-gel digestion for mass spectrometric characterization of proteins and proteomes Andrej Shevchenko, Henrik Tomas, J. Havli et al. · Nature Protocols | Dec 2006 | 5,186 |
| 33 | Skyline: an open source document editor for creating and analyzing targeted proteomics experiments Brendan MacLean, Daniela M. Tomazela, Nicholas Shulman et al. · Bioinformatics | 9 Feb 2010 | 5,063 |
| 34 | A Statistical Model for Identifying Proteins by Tandem Mass Spectrometry Alexey I. Nesvizhskii, Andrew Keller, Eugene Kolker et al. · Analytical Chemistry | 15 Jul 2003 | 5,023 |
| 35 | Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips Juri Rappsilber, Matthias Mann, Yasushi Ishihama · Nature Protocols | Aug 2007 | 5,019 |
| 36 | Empirical Statistical Model To Estimate the Accuracy of Peptide Identifications Made by MS/MS and Database Search Andrew Keller, Alexey I. Nesvizhskii, Eugene Kolker et al. · Analytical Chemistry | 12 Sep 2002 | 5,013 |
| 37 | Replica-exchange molecular dynamics method for protein folding Yuji Sugita, Yuko Okamoto · Chemical Physics Letters | Nov 1999 | 4,689 |
| 38 | Theoretical studies of enzymic reactions: Dielectric, electrostatic and steric stabilization of the carbonium ion in the reaction of lysozyme Arieh Warshel, Michael Levitt · Journal of Molecular Biology | May 1976 | 4,544 |
| 39 | Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging Reagents Philip L. Ross, Yulin Huang, Jason Marchese et al. · Molecular & Cellular Proteomics | 22 Sep 2004 | 4,464 |
| 40 | A cross-platform toolkit for mass spectrometry and proteomics Matthew Chambers et al. · Nature Biotechnology | Oct 2012 | 4,457 |
| 41 | The Human Plasma Proteome N. Leigh Anderson, Norman G. Anderson, Norman G. Anderson et al. · Molecular & Cellular Proteomics | Nov 2002 | 4,430 |
| 42 | The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes James T. Dodge, Carolyn D. Mitchell, Donald J. Hanahan · Archives of Biochemistry and Biophysics | 1963 | 4,421 |
| 43 | A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids D. Wessel, U.I. Flügge · Analytical Biochemistry | Apr 1984 | 4,146 |
| 44 | Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry Joshua Eric Elias, Steven P. Gygi · Nature Methods | 27 Feb 2007 | 4,142 |
| 45 | Silver stain for proteins in polyacrylamide gels: A modified procedure with enhanced uniform sensitivity James H. Morrissey · Analytical Biochemistry | Nov 1981 | 4,100 |
| 46 | MZmine 2: Modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data Tomáš Pluskal, Sandra D. Castillo, Alejandro Villar‐Briones et al. · BMC Bioinformatics | 23 Jul 2010 | 4,019 |
| 47 | Estimation of the molecular weights of proteins by Sephadex gel-filtration Patrick Andrews · Biochemical Journal | May 1964 | 3,905 |
| 48 | Determination of Alkali and Halide Monovalent Ion Parameters for Use in Explicitly Solvated Biomolecular Simulations In Suk Joung, Thomas E. Cheatham · The Journal of Physical Chemistry B | Jul 2008 | 3,735 |
| 49 | Molecular replacement with MOLREP Alexei A. Vagin, A. I. Teplyakov · Acta Crystallographica Section D Biological Crystallography | 21 Dec 2009 | 3,727 |
| 50 | 2016 update of the PRIDE database and its related tools Juan Antonio Vizcaíno, Attila Csordás, Noemí del‐Toro et al. · Nucleic Acids Research | 2 Nov 2015 | 3,695 |
Citations are OpenAlex's own counts, from its 2026-09-23 snapshot, so the ranking can differ from Google Scholar or Web of Science. A paper is listed for a field when OpenAlex files it under one of the field's topics; retracted papers and papers whose citation count cannot be traced to citing works are left out, and a preprint and the article it became are shown once. Release dates are OpenAlex's: where a source gave only a month or a year, that is what is shown. How the lists are chosen.