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Topic · Molecular Biology Techniques and Applications

Most cited papers in Molecular Biology Techniques and Applications

Molecular Biology Techniques and Applications

What are the most cited papers in Molecular Biology Techniques and Applications?

The most cited paper in Molecular Biology Techniques and Applications is "Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method" (Kenneth J. Livak, Thomas D. Schmittgen, 2001), with 184,956 citations. Next come "Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction" (63,261) and "Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction" (47,055).

Showing 50 of 100 papers. Every paper with more than 2,784 citations is listed.

Most cited papers, sortable by citations and release date
#Paper
1 Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method Kenneth J. Livak, Thomas D. Schmittgen · Methods Dec 2001 184,956
2 Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction P CHOMZYNSKI · Analytical Biochemistry Apr 1987 63,261
3 Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Piotr Chomczyński, Nicoletta Sacchi · Analytical Biochemistry Apr 1987 47,055
4 limma powers differential expression analyses for RNA-sequencing and microarray studies Matthew E. Ritchie, Belinda Phipson, Di Wu et al. · Nucleic Acids Research Open access 20 Jan 2015 44,530
5 A new mathematical model for relative quantification in real-time RT-PCR Michael Walter Pfaffl · Nucleic Acids Research Open access May 2001 35,329
6 Analyzing real-time PCR data by the comparative CT method Thomas D. Schmittgen, Kenneth J. Livak · Nature Protocols Jun 2008 27,138
7 Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology Open access 18 Jun 2002 20,347
8 Differential expression analysis for sequence count data Simon Anders, Wolfgang K. Huber · Genome biology Open access Oct 2010 16,645
9 The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments Stephen Andrew Bustin, Vladimı́r Beneš, Jeremy A. Garson et al. · Clinical Chemistry Open access 26 Feb 2009 16,583
10 Molecular portraits of human breast tumours Charles M. Perou, Thérese Sørlie, Michael B. Eisen et al. · Nature 17 Aug 2000 16,552
11 StringTie enables improved reconstruction of a transcriptome from RNA-seq reads Mihaela Pertea, Geo M. Pertea, Corina M. Antonescu et al. · Nature Biotechnology Open access 18 Feb 2015 16,085
12 Primer3 on the WWW for General Users and for Biologist Programmers Steve Rozen, Helen Skaletsky · Humana Press eBooks Book chapter 14 Nov 2003 15,110
13 Robust enumeration of cell subsets from tissue expression profiles Aaron M. Newman, Chih Long Liu, Michael Richard Green et al. · Nature Methods Open access 30 Mar 2015 14,493
14 Mapping and quantifying mammalian transcriptomes by RNA-Seq Ali Mortazavi, Brian A. Williams, Kenneth McCue et al. · Nature Methods Open access 30 May 2008 14,472
15 Salmon provides fast and bias-aware quantification of transcript expression Rob Patro, Geet Duggal, Michael I. Love et al. · Nature Methods Open access 6 Mar 2017 14,211
16 A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid K. Burton · Biochemical Journal Open access Feb 1956 14,144
17 Gene Expression Omnibus: NCBI gene expression and hybridization array data repository Ron Edgar · Nucleic Acids Research Open access 2002 14,110
18 RNA-Seq: a revolutionary tool for transcriptomics Zhong Wang, Mark Gerstein, Michael Snyder · Nature Reviews Genetics ReviewOpen access 18 Nov 2008 13,619
19 AFLP: a new technique for DNA fingerprinting Pieter Vos, René C. J. Hogers, Marjo Bleeker et al. · Nucleic Acids Research Open access 1995 12,461
20 Molecular Classification of Cancer: Class Discovery and Class Prediction by Gene Expression Monitoring Todd R. Golub, Donna K. Slonim, Pablo Tamayo et al. · Science 15 Oct 1999 11,660
21 Near-optimal probabilistic RNA-seq quantification Nicolas Bray, Harold Pimentel, Páll Melsted et al. · Nature Biotechnology 4 Apr 2016 11,277
22 Gene expression patterns of breast carcinomas distinguish tumor subclasses with clinical implications Thérese Sørlie, Charles M. Perou, Robert John Tibshirani et al. · Proceedings of the National Academy of Sciences Open access 11 Sep 2001 11,073
23 Exploration, normalization, and summaries of high density oligonucleotide array probe level data Rafael A. Irizarry · Biostatistics Apr 2003 10,831
24 Significance analysis of microarrays applied to the ionizing radiation response Virginia Goss Tusher, Robert John Tibshirani, Gilbert Chu · Proceedings of the National Academy of Sciences Open access 17 Apr 2001 10,744
25 GEPIA: a web server for cancer and normal gene expression profiling and interactive analyses Zefang Tang, Chenwei Li, Chenwei Li et al. · Nucleic Acids Research Open access 5 Apr 2017 10,731
26 Gene Selection for Cancer Classification using Support Vector Machines Isabelle Guyon, Jason Weston, S. Barnhill et al. · Machine Learning Open access 2002 10,052
27 Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling Ash A. Alizadeh et al. · Nature Feb 2000 10,021
28 A new technique for the assay of infectivity of human adenovirus 5 DNA Frank L. Graham, A.J. van der Eb · Virology Apr 1973 9,753
29 Gene expression profiling predicts clinical outcome of breast cancer Laura van ‘t Veer, Hongyue A. Dai, Marc J. van de Vijver et al. · Nature Open access 2002 9,661
30 Adjusting batch effects in microarray expression data using empirical Bayes methods William Evan Johnson, Cheng Li, Ariel Rabinovic · Biostatistics 21 Apr 2006 9,409
31 De novo transcript sequence reconstruction from RNA-seq using the Trinity platform for reference generation and analysis Brian J. Haas et al. · Nature Protocols Open access 11 Jul 2013 9,312
32 Gene ontology analysis for RNA-seq: accounting for selection bias Matthew D. Young, Matthew J. Wakefield, Gordon K. Smyth et al. · Genome biology Open access 4 Feb 2010 7,920
33 Site-directed mutagenesis by overlap extension using the polymerase chain reaction Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene Apr 1989 7,540
34 Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR Michael Walter Pfaffl · Nucleic Acids Research Open access May 2002 7,466
35 Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets Claus L. Andersen, Jens Ledet Jensen, Torben Falck Orntoft · Cancer Research Aug 2004 7,281
36 Sequencing technologies — the next generation Michael L. Metzker · Nature Reviews Genetics ReviewOpen access 8 Dec 2009 7,133
37 Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology Erko Stackebrandt, Brett M. Goebel · INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY Oct 1994 7,129
38 voom: precision weights unlock linear model analysis tools for RNA-seq read counts Charity W. Law, Yunshun Chen, Wei Shi et al. · Genome biology Open access 3 Feb 2014 6,910
39 Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction Jian Ye, George Coulouris, Irena Zaretskaya et al. · BMC Bioinformatics Open access 18 Jun 2012 6,397
40 Differential expression analysis of multifactor RNA-Seq experiments with respect to biological variation Davis James McCarthy, Yunshun Chen, Gordon K. Smyth · Nucleic Acids Research Open access 28 Jan 2012 5,910
41 affy—analysis of Affymetrix GeneChip data at the probe level Laurent Gautier, Leslie Cope, Benjamin M. Bolstad et al. · Bioinformatics Open access 11 Feb 2004 5,481
42 Proposed minimum reporting standards for chemical analysis Lloyd W. Sumner et al. · Metabolomics Open access 11 Sep 2007 5,359
43 Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations Michael Walter Pfaffl, Aleš Tichopád, Christian Prgomet et al. · Biotechnology Letters Mar 2004 5,178
44 The use of DAPI for identifying and counting aquatic microflora1 Karen Glaus Porter, Yvette S. Feig · Limnology and Oceanography Open access Sep 1980 5,103
45 Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction Peng Liang, Arthur B. Pardee · Science 14 Aug 1992 5,040
46 Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. Paul Thomas Matsudaira · Journal of Biological Chemistry Open access Jul 1987 4,982
47 DEGseq: an R package for identifying differentially expressed genes from RNA-seq data Likun Wang, Zhixing Feng, Xi Wang et al. · Bioinformatics Open access 24 Oct 2009 4,944
48 Screening λgt Recombinant Clones by Hybridization to Single Plaques in Situ W. David Benton, Ronald W. Davis · Science 8 Apr 1977 4,662
49 Full-length RNA-seq from single cells using Smart-seq2 Simone Picelli, Omid R. Faridani, Åsa K. Björklund et al. · Nature Protocols 2014 4,639
50 Next-generation DNA sequencing Jay Shendure, Hanlee P. Ji · Nature Biotechnology Oct 2008 4,546

Citations are OpenAlex's own counts, from its 2026-09-23 snapshot, so the ranking can differ from Google Scholar or Web of Science. A paper is listed for a field when OpenAlex files it under one of the field's topics; retracted papers and papers whose citation count cannot be traced to citing works are left out, and a preprint and the article it became are shown once. Release dates are OpenAlex's: where a source gave only a month or a year, that is what is shown. How the lists are chosen.