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Most cited papers in Protein purification and stability

Protein purification and stability

What are the most cited papers in Protein purification and stability?

The most cited paper in Protein purification and stability is "The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis" (Karolin Weber, Mary Osborn, 1969), with 20,350 citations. Next come "DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*" (18,971) and "Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction" (12,981).

Showing 50 of 100 papers. Every paper with more than 980 citations is listed.

Most cited papers, sortable by citations and release date
#Paper
1 The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Karolin Weber, Mary Osborn · Journal of Biological Chemistry Open access Aug 1969 20,350
2 DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS* Baruch J. Davis · Annals of the New York Academy of Sciences Dec 1964 18,971
3 Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction Yung-Chi Cheng, William H. Prusoff · Biochemical Pharmacology Dec 1973 12,981
4 Immunochemical quantitation of antigens by single radial immunodiffusion G Mancini, Angelo Oscar Carbonara, Joseph F. Heremans · Immunochemistry Sep 1965 9,209
5 A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures Robert G. Martin, Bruce N. Ames · Journal of Biological Chemistry Open access May 1961 5,579
6 Modeling and comparison of dissolution profiles Paulo C. Costa, José Manuel Sousa Lobo · European Journal of Pharmaceutical Sciences May 2001 5,484
7 Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips Juri Rappsilber, Matthias Mann, Yasushi Ishihama · Nature Protocols Aug 2007 5,019
8 Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. Paul Thomas Matsudaira · Journal of Biological Chemistry Open access Jul 1987 4,982
9 Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies Carl‐Bertil Laurell · Analytical Biochemistry Apr 1966 4,438
10 A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids D. Wessel, U.I. Flügge · Analytical Biochemistry Apr 1984 4,146
11 Size-Distribution Analysis of Macromolecules by Sedimentation Velocity Ultracentrifugation and Lamm Equation Modeling Peter Schuck · Biophysical Journal Open access Mar 2000 4,003
12 Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels Arnold L. Shapiro, Eladio Viñuela, Jacob V. Maizel · Biochemical and Biophysical Research Communications Sep 1967 3,779
13 A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250 J. JAMES SEDMAK, Sidney E. Grossberg · Analytical Biochemistry May 1977 3,041
14 Coated Charcoal Immunoassay of Insulin Victor D. Herbert, Kam‐Seng Lau, Chester W. Gottlieb et al. · The Journal of Clinical Endocrinology & Metabolism Oct 1965 2,999
15 Rapid Isolation of Antigens from Cells with A Staphylococcal Protein A-Antibody Adsorbent: Parameters of the Interaction of Antibody-Antigen Complexes with Protein A Steven Kessler · The Journal of Immunology Dec 1975 2,949
16 Protein Purification by Affinity Chromatography Pedro Cuatrecasas · Journal of Biological Chemistry Open access Jun 1970 2,803
17 Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-Sepharose Peter L. Ey, Stephen J. Prowse, Charles R. Jenkin · Immunochemistry Jul 1978 2,697
18 Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250 Volker NEUHOFF, Norbert Arold, Dieter Taube et al. · Electrophoresis 1988 2,692
19 A short amino acid sequence able to specify nuclear location Daniel Kalderon, Bruce Roberts, William David Richardson et al. · Cell Dec 1984 2,587
20 Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polycrylamide to nitrocellulose Jan Kyhse‐Andersen · Journal of Biochemical and Biophysical Methods Dec 1984 2,521
21 The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability F. Niesen, Helena Berglund, Masoud Vedadi · Nature Protocols Sep 2007 2,481
22 Phage antibodies: filamentous phage displaying antibody variable domains John McCafferty, Andrew D. Griffiths, Greg P. Winter et al. · Nature Dec 1990 2,430
23 Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases Lewis M. Siegel, Kenneth J. Monty · Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Feb 1966 2,239
24 Serum Albumin Theodore Peters · Advances in protein chemistry Book chapter 1985 2,180
25 Pharmaceutical Applications of Cyclodextrins. 1. Drug Solubilization and Stabilization Þorsteinn Loftsson, Marcus Eli Brewster · Journal of Pharmaceutical Sciences Oct 1996 2,168
26 Single-pot, solid-phase-enhanced sample preparation for proteomics experiments Christopher S. Hughes, Sophie Moggridge, Torsten Müller et al. · Nature Protocols 16 Nov 2018 2,133
27 Protein therapeutics: a summary and pharmacological classification Benjamin Leader, Quentin J. Baca, David E. Golan · Nature Reviews Drug Discovery Review 21 Dec 2007 2,127
28 Metal chelate affinity chromatography, a new approach to protein fractionation Jerker O. Porath, Jan Carlsson, Ingmar Olsson et al. · Nature Dec 1975 2,068
29 Determination of free amino groups in proteins by trinitrobenzenesulfonic acid Ahmed F.S.A. Habeeb · Analytical Biochemistry Mar 1966 2,043
30 Human serum albumin: From bench to bedside Gabriella Fanali, Alessandra di Masi, Viviana Trezza et al. · Molecular Aspects of Medicine 30 Dec 2011 1,959
31 A simplified method for cyanogen bromide activation of agarose for affinity chromatography S. Cervera March, Indu Parikh, Pedro Cuatrecasas · Analytical Biochemistry Jul 1974 1,942
32 Engineered antibody fragments and the rise of single domains Philipp Holliger, Peter J. Hudson · Nature Biotechnology Sep 2005 1,939
33 Protein biomarker discovery and validation: the long and uncertain path to clinical utility Nader Rifai, Michael A. Gillette, Steven A. Carr · Nature Biotechnology Aug 2006 1,876
34 Adenovirus-Associated Virus Vector–Mediated Gene Transfer in Hemophilia B Amit C. Nathwani et al. · New England Journal of Medicine Open access 10 Dec 2011 1,875
35 Novel hydrophilic chitosan-polyethylene oxide nanoparticles as protein carriers Pilar Calvo, C. Remu��n-L�pez, J. L. Vila-Jato et al. · Journal of Applied Polymer Science 3 Jan 1997 1,869
36 Glutaraldehyde: Behavior in Aqueous Solution, Reaction with Proteins, and Application to Enzyme Crosslinking Isabelle Migneault, Catherine Dartiguenave, Michel J. Bertrand et al. · BioTechniques Open access Nov 2004 1,821
37 A simplified method for the quantitative assay of small amounts of protein in biologic material George R. Schacterle, Robert L. Pollack · Analytical Biochemistry Feb 1973 1,800
38 Eukaryotic proteins expressed in Escherichia coli: An improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase Kun‐Liang Guan, Jack E. Dixon · Analytical Biochemistry Feb 1991 1,768
39 Enzyme-Linked Immunosorbent Assay, Elisa Eva Olsson Engvall, Peter Perlmann · The Journal of Immunology Jul 1972 1,755
40 Targeted Drug Delivery with Polymers and Magnetic Nanoparticles: Covalent and Noncovalent Approaches, Release Control, and Clinical Studies Karel Ulbrich, Kateřina Holá, Vladimír Šubr et al. · Chemical Reviews ReviewOpen access 25 Apr 2016 1,738
41 Electroimmuno Assay C.‐B. Laurell · Scandinavian Journal of Clinical and Laboratory Investigation 1972 1,700
42 Antibodies to major histocompatibility antigens produced by hybrid cell lines G. Galfré, Sebastian Howe, César Milstein et al. · Nature Apr 1977 1,699
43 Overcoming the challenges in administering biopharmaceuticals: formulation and delivery strategies Samir Mitragotri, Paul A. Burke, Róbert Langer · Nature Reviews Drug Discovery ReviewOpen access 8 Aug 2014 1,696
44 Understanding protein adsorption phenomena at solid surfaces Michael Rabe, Dorinel Verdes, Stefan Seeger · Advances in Colloid and Interface Science Open access 13 Jan 2011 1,667
45 Solid dispersion of poorly water‐soluble drugs: Early promises, subsequent problems, and recent breakthroughs Abu T.M. Serajuddin · Journal of Pharmaceutical Sciences Oct 1999 1,650
46 Size and Shape of Protein Molecules at the Nanometer Level Determined by Sedimentation, Gel Filtration, and Electron Microscopy Harold Erickson · Biological Procedures Online Open access 15 May 2009 1,637
47 Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. James S. Huston, Dennis J. Levinson, Meredith Mudgett-Hunter et al. · Proceedings of the National Academy of Sciences Open access Aug 1988 1,602
48 Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification Keith R. Yamamoto, Bruce Alberts, Rolf H. Benzinger et al. · Virology Open access Mar 1970 1,601
49 Freeze-drying of nanoparticles: Formulation, process and storage considerations☆ Wassim Abdelwahed, Ghania Degobert, Serge Stainmesse et al. · Advanced Drug Delivery Reviews 7 Oct 2006 1,595
50 Reshaping human antibodies for therapy Lutz Riechmann, Mike Clark, Herman Waldmann et al. · Nature Mar 1988 1,565

Citations are OpenAlex's own counts, from its 2026-09-23 snapshot, so the ranking can differ from Google Scholar or Web of Science. A paper is listed for a field when OpenAlex files it under one of the field's topics; retracted papers and papers whose citation count cannot be traced to citing works are left out, and a preprint and the article it became are shown once. Release dates are OpenAlex's: where a source gave only a month or a year, that is what is shown. How the lists are chosen.