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Topic · Molecular Biology

Protein purification and stability

Protein purification and stability is a research topic within Molecular Biology. Science Explorer counts 32k research works in it since 1950. 9.9% of them reached the world's top 10% most cited for their field and year.

This cluster of papers focuses on the analysis, mechanisms, and control of protein aggregation in biopharmaceuticals, particularly recombinant proteins and therapeutic antibodies. It also delves into the stability, chromatographic purification, immunogenicity, and refolding of these proteins, as well as the use of sedimentation velocity for size-distribution analysis.

  • Protein Aggregation
  • Biopharmaceuticals
  • Recombinant Proteins
  • Therapeutic Antibodies
  • Stability
  • Chromatography
  • Immunogenicity
  • Refolding
  • Monoclonal Antibodies
  • Sedimentation Velocity
Research works
32k
fractional, since 1950
In the world top 10%
3.2k
per year above
Top-10% rate
9.9%
share of its works in the world top 10%
Growth, 2018-21 → 2022-25
+1%
the tick is no change
Largest producer
United States
33% of the 10 largest, 2022-now
Protein purification and stability

Which countries lead Protein purification and stability research?

By volume, the United States and China publish the most (853 and 484 works in 2022-now).

By volume, 2022-now

  1. 1 United States 853 works
  2. 2 China 484 works
  3. 3 India 293 works
  4. 4 Germany 267 works
  5. 5 Japan 188 works
  6. 6 United Kingdom 156 works
  7. 7 France 113 works
  8. 8 Russia 105 works
  9. 9 Switzerland 75 works
  10. 10 Canada 68 works

How concentrated that is

The same countries as shares of everything the list above accounts for. A node where two countries do two thirds of the work and one spread evenly across twelve read alike as a ranking and not at all alike here.

United States: 32.8%China: 18.6%India: 11.2%Germany: 10.3%6 others listed: 27.1%33%largest
United States853 · 32.8%China484 · 18.6%India293 · 11.2%Germany267 · 10.3%6 others listed705 · 27.1%

Shares of the rows listed above, not of the whole node.

Which institutions lead Protein purification and stability research?

By volume in 2022-now, Merck & Co., Inc., Rahway, NJ, USA (United States) publishes the most Protein purification and stability research, followed by Russian Academy of Sciences and AstraZeneca (United Kingdom).

By volume, 2022-now

  1. 1 Merck & Co., Inc., Rahway, NJ, USA (United States)United States 46 works
  2. 2 Russian Academy of SciencesRussia 38 works
  3. 3 AstraZeneca (United Kingdom)United Kingdom 35 works
  4. 4 Centre National de la Recherche ScientifiqueFrance 28 works
  5. 5 Indian Institute of Technology DelhiIndia 27 works
  6. 6 Roche (Switzerland)Switzerland 27 works
  7. 7 WuXi AppTec (China)China 27 works
  8. 8 Bristol-Myers Squibb (United States)United States 26 works
  9. 9 Boehringer Ingelheim (Germany)Germany 25 works
  10. 10 Regeneron (United States)United States 24 works

Who are the leading researchers in Protein purification and stability?

The most-cited researchers publishing on Protein purification and stability include Christopher J L Murray, Matthias Mann and Róbert Langer.

  1. 1 Christopher J L Murray United States 583k citations
  2. 2 Matthias Mann Germany 308k citations
  3. 3 Róbert Langer United States 303k citations
  4. 4 Ulrich K. Laemmli Switzerland 285k citations
  5. 5 George M. Whitesides United States 267k citations
  6. 6 Mark Gerstein United States 250k citations

Ranked by citations received across their whole record, among researchers with at least three works on this topic.

What are the most cited papers in Protein purification and stability?

The most cited paper in Protein purification and stability is "The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis" (Karolin Weber, Mary Osborn, 1969), with 20,350 citations. Next come "DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*" (18,971) and "Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction" (12,981).

Most cited papers
#PaperReleasedCitations
1 The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Karolin Weber, Mary Osborn · Journal of Biological Chemistry Open access Aug 1969 20,350
2 DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS* Baruch J. Davis · Annals of the New York Academy of Sciences Dec 1964 18,971
3 Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction Yung-Chi Cheng, William H. Prusoff · Biochemical Pharmacology Dec 1973 12,981
4 Immunochemical quantitation of antigens by single radial immunodiffusion G Mancini, Angelo Oscar Carbonara, Joseph F. Heremans · Immunochemistry Sep 1965 9,209
5 A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures Robert G. Martin, Bruce N. Ames · Journal of Biological Chemistry Open access May 1961 5,579
All 100 most cited papers, with filters and sorting

Where is Protein purification and stability research done?

The largest centres of Protein purification and stability research in 2022-now are Shanghai (China), Beijing (China), Tokyo (Japan) and Moscow (Russia). Among places with at least 20 works in it, it is an unusually large share of all research in Tarrytown, Rahway and Thousand Oaks.

Largest cities, 2022-now

  1. 1 ShanghaiChina 77 works
  2. 2 BeijingChina 77 works
  3. 3 TokyoJapan 63 works
  4. 4 MoscowRussia 58 works
  5. 5 New YorkUnited States 51 works
  6. 6 LondonUnited Kingdom 48 works
  7. 7 RahwayUnited States 46 works
  8. 8 ParisFrance 45 works
  9. 9 New DelhiIndia 42 works
  10. 10 ViennaAustria 33 works

Where it is the local speciality

  1. TarrytownUS · 23.9 works190×
  2. RahwayUS · 45.9 works153×
  3. Thousand OaksUS · 20.7 works84×
  4. BaselCH · 30.8 works14×
← less than its size predicts1×more →

Location quotient: how much more of its research is in Protein purification and stability than the world average.

See Protein purification and stability on the map

Where is the best place to study Protein purification and stability?

Among universities, judged by research, University of Delaware, Indian Institute of Technology Delhi and Henan Medical University score highest, combining excellence, specialisation, size, growth and international reach. Research strength is one signal when choosing where to study; it does not measure teaching.

0%10%20%30%mean 16.07%fractional works in this field (log) →share of work in the world top 10% →Henan Medical Univer…Massachusetts Instit…University of DelawareIndian Institute of …
above the meannear itbelow it

One dot per university in the first ten of the table below, at the default weights. The upper left is the interesting corner: small places doing unusually strong work.

Excellenceshare of its work in the world top 10%, 2022-now and all time averaged
40%
Specialisationhow much of its work is in this field, counted up to 10× the world share
10%
Sizeits output in this field, on a log scale
30%
Growthoutput growth, counted from -20% to +20%
10%
Internationalshare of its work with a co-author abroad
10%
Each slider sets a weight; the share it gets of the score is beside it.
#UniversityScoreTop 10%SpecialisationWorksGrowth
1University of DelawareUnited States 65.416.2%13.8×12 +32.6%
2Indian Institute of Technology DelhiIndia 61.99.5%17.3×28 +2.8%
3Henan Medical UniversityChina 60.026.4%30.4×8 +269.2%
4Massachusetts Institute of TechnologyUnited States 57.819.7%5.1×11 -24.0%
5University College LondonUnited Kingdom 55.514.2%3.1×15 +18.2%
6BOKU UniversityAustria 53.39.3%42.3×14 -3.4%
7Imperial College LondonUnited Kingdom 50.419.5%3.9×12 -3.4%
8Technical University of DenmarkDenmark 49.813.8%7.0×11 +70.4%
9Purdue University West LafayetteUnited States 47.611.7%5.5×16 -22.0%
10The University of Texas at AustinUnited States 46.320.4%3.4×9 -6.2%

Universities only. The score blends excellence (40%), size (30%), specialisation (10%), growth (10%), international reach (10%), 2022-now; growth compares 2018-21 with 2022-25 and counts up to +20%, since a jump from a small base is mostly noise.

Every institution in Protein purification and stability, country by country

Is Protein purification and stability research growing?

Output in 2022-2025 was 1% higher than in 2018-2021, peaking in 2025. The fastest-growing topics are Protein purification and stability.

still counting19801990200020102020
grewheldshrank

The same series as a ribbon: one cell per year, darker for more. The line above answers how much; this answers when.

Its share of all research

Growing output can still be a shrinking share: all of science grows. This is Protein purification and stability as a percentage of every work published that year, 0.015% in 2024.

still counting0.050%0.10%Protein purification and stability0.014% in 202519801990200020102020

Fractional works of Protein purification and stability over all fractional works that year. How the balance of science has shifted.

How much of it reaches the world top 10%

Year by year.

still counting5%10%15%Protein purification and stability10.0% in 2025200020102020

Of each year's works in it, the share that reached the world's 10% most cited for their field and year. A field is the world for its own topics, so there is no other line to hold it against.

Which topics inside it are moving

Growth and decline on one axis around a shared zero. Two lists side by side hide the thing that matters: whether the growth dwarfs the decline, or the other way round.

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