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Topic · Biophysics

Advanced Fluorescence Microscopy Techniques

Advanced Fluorescence Microscopy Techniques is a research topic within Biophysics. Science Explorer counts 29k research works in it since 1950. 9.8% of them reached the world's top 10% most cited for their field and year.

This cluster of papers covers a wide range of advances in fluorescence microscopy techniques, including super-resolution imaging, single-molecule imaging, fluorescence correlation spectroscopy, nanoscopy, and the development of various fluorescent probes such as calcium indicators and photoactivatable probes. It also includes applications of these techniques in studying cellular structures, protein-protein interactions, and neuronal activity.

  • Fluorescent Proteins
  • Super-Resolution Imaging
  • Single-Molecule Imaging
  • Fluorescence Correlation Spectroscopy
  • Nanoscopy
  • Calcium Indicators
  • Photoactivatable Probes
  • Light-Sheet Microscopy
  • FRET Microscopy
  • Multiphoton Microscopy
Research works
29k
fractional, since 1950
In the world top 10%
2.9k
per year above
Top-10% rate
9.8%
share of its works in the world top 10%
Growth, 2018-21 → 2022-25
-5%
the tick is no change
Largest producer
United States
35% of the 10 largest, 2022-now
Advanced Fluorescence Microscopy Techniques

Which countries lead Advanced Fluorescence Microscopy Techniques research?

By volume, the United States and China publish the most (1.5k and 1.1k works in 2022-now).

By volume, 2022-now

  1. 1 United States 1.5k works
  2. 2 China 1.1k works
  3. 3 Germany 449 works
  4. 4 United Kingdom 291 works
  5. 5 France 278 works
  6. 6 Japan 259 works
  7. 7 South Korea 146 works
  8. 8 India 144 works
  9. 9 Italy 134 works
  10. 10 Canada 116 works

How concentrated that is

The same countries as shares of everything the list above accounts for. A node where two countries do two thirds of the work and one spread evenly across twelve read alike as a ranking and not at all alike here.

United States: 34.5%China: 24.9%Germany: 10.0%United Kingdom: 6.5%6 others listed: 24.0%35%largest
United States1,549 · 34.5%China1,119 · 24.9%Germany449 · 10.0%United Kingdom291 · 6.5%6 others listed1,077 · 24.0%

Shares of the rows listed above, not of the whole node.

Which institutions lead Advanced Fluorescence Microscopy Techniques research?

By volume in 2022-now, Centre National de la Recherche Scientifique publishes the most Advanced Fluorescence Microscopy Techniques research, followed by Chinese Academy of Sciences and Harvard University.

By volume, 2022-now

  1. 1 Centre National de la Recherche ScientifiqueFrance 125 works
  2. 2 Chinese Academy of SciencesChina 109 works
  3. 3 Harvard UniversityUnited States 51 works
  4. 4 Max Planck SocietyGermany 49 works
  5. 5 Zhejiang UniversityChina 37 works
  6. 6 Shenzhen UniversityChina 37 works
  7. 7 Russian Academy of SciencesRussia 37 works
  8. 8 National Institutes of HealthUnited States 35 works
  9. 9 Tsinghua UniversityChina 33 works
  10. 10 Peking UniversityChina 32 works

Who are the leading researchers in Advanced Fluorescence Microscopy Techniques?

The most-cited researchers publishing on Advanced Fluorescence Microscopy Techniques include Eric S. Lander, Guido Kroemer and Karl John Friston.

  1. 1 Eric S. Lander United States 524k citations
  2. 2 Guido Kroemer France 386k citations
  3. 3 Karl John Friston United Kingdom 310k citations
  4. 4 Matthias Mann Germany 308k citations
  5. 5 Róbert Langer United States 303k citations
  6. 6 Todd R. Golub United States 283k citations
  7. 7 Wei Huang China 267k citations
  8. 8 Gordon Brent Mills United States 265k citations
  9. 9 Ben Zhong Tang China 261k citations
  10. 10 Hans C. Clevers Netherlands 260k citations

Ranked by citations received across their whole record, among researchers with at least three works on this topic.

What are the most cited papers in Advanced Fluorescence Microscopy Techniques?

The most cited paper in Advanced Fluorescence Microscopy Techniques is "Comprehensive Integration of Single-Cell Data" (Tim Stuart, Andrew W. Butler, Paul Hoffman et al., 2019), with 17,471 citations. Next come "Fast, sensitive and accurate integration of single-cell data with Harmony" (11,339) and "Two-Photon Laser Scanning Fluorescence Microscopy" (9,531).

Most cited papers
#PaperReleasedCitations
1 Comprehensive Integration of Single-Cell Data Tim Stuart, Andrew W. Butler, Paul Hoffman et al. · Cell Open access Jun 2019 17,471
2 Fast, sensitive and accurate integration of single-cell data with Harmony Ilya Korsunsky, Nghia Millard, Jean Fan et al. · Nature Methods Open access 18 Nov 2019 11,339
3 Two-Photon Laser Scanning Fluorescence Microscopy Winfried T. Denk, James H. Strickler, Watt W. Webb · Science 6 Apr 1990 9,531
4 Imaging Intracellular Fluorescent Proteins at Nanometer Resolution Eric Betzig, George H. Patterson, Rachid Sougrat et al. · Science 10 Aug 2006 9,061
5 Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM) Michael J. Rust, Mark Bates, Xiaowei Zhuang · Nature Methods Open access 9 Aug 2006 8,412
All 100 most cited papers, with filters and sorting

Where is Advanced Fluorescence Microscopy Techniques research done?

The largest centres of Advanced Fluorescence Microscopy Techniques research in 2022-now are Beijing (China), Paris (France), Shanghai (China) and Tokyo (Japan). Among places with at least 20 works in it, it is an unusually large share of all research in Jena and Göttingen.

Largest cities, 2022-now

  1. 1 BeijingChina 234 works
  2. 2 ParisFrance 127 works
  3. 3 ShanghaiChina 90 works
  4. 4 TokyoJapan 76 works
  5. 5 LondonUnited Kingdom 72 works
  6. 6 ShenzhenChina 71 works
  7. 7 HangzhouChina 71 works
  8. 8 SeoulSouth Korea 67 works
  9. 9 NanjingChina 65 works
  10. 10 CambridgeUnited States 56 works

Where it is the local speciality

  1. JenaDE · 28.1 works12×
  2. GöttingenDE · 20.8 works8.5×
← less than its size predicts1×more →

Location quotient: how much more of its research is in Advanced Fluorescence Microscopy Techniques than the world average.

See Advanced Fluorescence Microscopy Techniques on the map

Where is the best place to study Advanced Fluorescence Microscopy Techniques?

Among universities, judged by research, Harvard University, Peking University and University of California, Berkeley score highest, combining excellence, specialisation, size, growth and international reach. Research strength is one signal when choosing where to study; it does not measure teaching.

0%10%20%30%mean 17.25%fractional works in this field (log) →share of work in the world top 10% →California Institute…Peking UniversityHarvard UniversityUniversity of Califo…
above the meannear itbelow it

One dot per university in the first ten of the table below, at the default weights. The upper left is the interesting corner: small places doing unusually strong work.

Excellenceshare of its work in the world top 10%, 2022-now and all time averaged
40%
Specialisationhow much of its work is in this field, counted up to 10× the world share
10%
Sizeits output in this field, on a log scale
30%
Growthoutput growth, counted from -20% to +20%
10%
Internationalshare of its work with a co-author abroad
10%
Each slider sets a weight; the share it gets of the score is beside it.
#UniversityScoreTop 10%SpecialisationWorksGrowth
1Harvard UniversityUnited States 75.915.4%3.4×51 -16.1%
2Peking UniversityChina 72.020.7%3.2×32 +27.6%
3University of California, BerkeleyUnited States 69.115.3%6.7×30 +2.6%
4California Institute of TechnologyUnited States 66.420.8%10.6×18 -2.6%
5École Polytechnique Fédérale de LausanneSwitzerland 66.120.7%10.9×21 -34.4%
6Massachusetts Institute of TechnologyUnited States 65.113.9%8.5×28 -1.6%
7Stanford UniversityUnited States 64.418.1%3.8×26 -43.7%
8Shenzhen UniversityChina 61.09.7%10.6×37 +8.1%
9University of Chinese Academy of SciencesChina 59.913.0%3.6×28 +48.5%
10University of GenevaSwitzerland 59.724.9%4.2×8 +3.8%

Universities only. The score blends excellence (40%), size (30%), specialisation (10%), growth (10%), international reach (10%), 2022-now; growth compares 2018-21 with 2022-25 and counts up to +20%, since a jump from a small base is mostly noise.

Every institution in Advanced Fluorescence Microscopy Techniques, country by country

Is Advanced Fluorescence Microscopy Techniques research growing?

Output in 2022-2025 was 5% lower than in 2018-2021, peaking in 2021.

still counting19801990200020102020
grewheldshrank

The same series as a ribbon: one cell per year, darker for more. The line above answers how much; this answers when.

Its share of all research

Growing output can still be a shrinking share: all of science grows. This is Advanced Fluorescence Microscopy Techniques as a percentage of every work published that year, 0.022% in 2024.

still counting0.020%0.040%Advanced Fluorescence Microscopy Techniques0.020% in 202519801990200020102020

Fractional works of Advanced Fluorescence Microscopy Techniques over all fractional works that year. How the balance of science has shifted.

How much of it reaches the world top 10%

Year by year.

still counting5%10%15%Advanced Fluorescence Microscopy Techniques9.8% in 2025200020102020

Of each year's works in it, the share that reached the world's 10% most cited for their field and year. A field is the world for its own topics, so there is no other line to hold it against.

Which topics inside it are moving

Growth and decline on one axis around a shared zero. Two lists side by side hide the thing that matters: whether the growth dwarfs the decline, or the other way round.

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